BIELSCHOWSKY METHOD
来自 : 蚂蚁淘
SECTIONS8micronparaffinwaxsections.
SOLUTIONS
1.SilverA-20%silvernitrate
2.Reducer(Makefresh)
- Formaldehyde50ml
- AbsoluteAlcohol200ml
- Distilledwater750ml
3.SilverB
- 20%Silvernitrate20ml
- 95%Alcohol20ml
- Addammoniauntilprecipitateformedisredissolved
- Add10moredropsofammonia
4.5%Sodiumthiosulphate5.0.1%Goldchloride
METHOD
- Takesectionstodistilledwater
- PlaceinsolutionAfor2-3hours
- Washinreducerfor2-5minutes
- Washin10%alcoholfor2minutes
- PlaceinsolutionBfor1-2minutes
- Washinaconstantflowofreducerandleavefor3-5minutes
- Washindistilledwater
- Tone0.1%goldchloridefor2minutes
- Washindistilledwater
- Fixin5%sodiumthiosulphatefor2minutes
- Washintapwater
- Dehydrate,clear.MountsectionsinDPX
RESULTSNeuritesaroundsenileplaques,neurofibrillarytangles-Black.
NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.
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