easYmer HLA-B*46:01 MHC Tetramers Kit
easYmer HLA-B*46:01 MHC Tetramers Developed and Manufactured by immunAware
Materials Included:
The easYmer kit contains a peptide receptive preparation of HLA, a folding buffer, a positive control peptide for HLA-complex folding. The exact identities of these components are given below:
- easYmer: HLA-B*46:01 – peptide receptive, biotinylated in Tris/Maleate pH 7 with 30% Glycerol
- Folding Buffer: Tris/Maleate pH7
- Peptide: SAFGGGNAF, a stable HLA-B*46:01 binder. Positive control for evaluation analysis of peptide-HLA folding.
For Research Use Only
Available Sizes:
- 20 Tests (Sample-size)
- 50 Tests (Standard Size)
- 150 Tests
- 500 Tests
Key Benefits of easYmer® MHC Tetramers
- Ready-to-use
- One step loading
- Completely flexible and customizable*
- Biotinylated
- No special equipment needed
- Long shelf-life
*For Custom Tetramer Production, please Contact Us for more information
Assay Principle
This easYmer HLA-B*46:01 MHC Tetramers protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß2-microglobulin (ß2m? light chain subunit of recombinant HLA Class I (HLA-I) comlexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.
Products Related to easYmer HLA-B*46:01 MHC Tetramers
easYmer HLA-B*55:01 MHC Tetramers Kit
easYmer HLA-B*57:02 MHC Tetramers Kit
easYmer HLA-B*15:01 MHC Tetramers Kit
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小弟好苦恼啊，求各位老师指点迷津！
版主shitou0307留言:
鼓励
就是价格高一般都都2000左右，普通厂家国产一般在200左右，具体看参数，磁力搅拌器一以电磁物理特性工作，所以一般个人用推荐就买普通国产就行，对于实验室要求较高的大型实验室，可购买进口类，不过现在很多国产也有很不错的价格一般在进口半价左右。
第一、在第一次使用时，先对照仪器说明书检查仪器所带配件是否齐全，譬如搅拌子、电源线等；
第二、调速时应由低速逐步调至高速，最好不要高速档直接起动，以免搅拌子不同步，引起跳动；
第三、中速运转可延长搅拌器的使用寿命；
第四、仪器应保持清洁干燥，尤其不要使溶液进入机内；
第五、搅拌时如果发现搅拌子跳动或不搅拌，请检查一下烧杯是否平稳，位置是否正；
第六、不搅拌时不能加热，不工作时应切断电源；
第七、使用时最好能够接上地线。
参考资料:Hi.baidu/=。www.jingda17.cn。DI
如下图示
问题是：有没有驱动和罐体分开的，像实验室用的那种，如下图：
后者使用移动配液罐，方便灭菌。
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