Highlights
- Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps, etc.
- Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, or vacuum source.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Description
| Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
|---|---|
| Elution Volume | ≥ 150 µl |
| Equipment | Microcentrifuge and centrifuge or vacuum source. |
| Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| Sample Source | DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc. |
| Size Range | 50 bp to 23 kb |
| Yield | ≤ 100 µg total DNA can be recovered.For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q2: How to process naked DNA stored in DNA/RNA Shield?
Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).
Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?
The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How many times can columns be reloaded?
We recommend no more than 5 times as binding efficiency might decrease.
Q6: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
“This kit performed as described. I was able to obtain good quality and yield of the DNA -better than with a couple of competitor"s kits.”
-Kathleen B. (SUNY-ESF)
“The simple steps and washing instructions were excellent. DNA was ready for use for PCR and gave strong results.”
-Tyler C.
“It was a very simple procedure and it gave a concentration ten times the original amount.”
-Kimberly M. (University of Pennsylvania)
Read More| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml | $60.00 | |
| D4004-1-L | DNA Binding Buffer | 100 ml | $57.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
| D4003-1-L | DNA Binding Buffer | 50 ml | $33.00 | |
| D3004-4-10 | DNA Elution Buffer | 10 ml | $14.00 | |
| C1001-1000 | Collection Tubes | 1000 Pack | $90.00 | |
| C1001-500 | Collection Tubes | 500 Pack | $52.00 | |
| C1016-25 | Zymo-Spin V Columns w/ Reservoir | 25 Pack | $67.00 | |
| C1016-50 | Zymo-Spin V Columns w/ Reservoir | 50 Pack | $105.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 |
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