SARS-CoV-2 Spike RBD Monoclonal Antibody
Human IgG Monoclonal Antibody.The monoclonal SARS-CoV-2 (2019-nCoV) spike antibody (S protein antibody, spike S1 antibody) was produced in mammalian cells.Specificity/Sensitivity: The monoclonal SARS-CoV-2 (2019-nCoV) spike RBD antibody binds to SARS-CoV-2 and SARS-CoV spike RBD, S protein, and S1 subunit.Applications: ELISA 1:10000, Flow Cytometry 1:500. It might be used as the human IgG antibody positive control for serological assays to detect SARS-CoV and SARS-CoV-2 infection.Form of Antibody: 1 mg/ml in PBS, pH 7.4.Clone: 25G1.Isotype: hIgG1, H+L.Purity: >95% by SDS-PAGE under reducing conditions.Shipping: The monoclonal SARS-CoV-2 spike RBD antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.
1 month from date of receipt, 2 to 8°C as supplied.
Background
The receptor binding domain (RBD) in the S1 subunit of the SARS-CoV-2 (2019-nCoV) spike (S) glycoprotein plays the most important roles in viral attachment, fusion and entry. The SARS-CoV and SARS-CoV-2 S proteins mediate viral entry into host cells by binding to a host receptor, angiotensin-converting enzyme 2 (ACE2), through the receptor-binding domain (RBD) in the S1 subunit, and then fusing the viral and host membranes through the S2 subunit. The ACE2-binding affinity of the Spike RBD of SARS-CoV-2 is 10- to 20-fold higher than that of SARS-CoV.
The SARS-CoV-2 S protein, especially its S1 subunit and RBD, has become a popular target of antibodies for diagnostics test, and proteins and antibodies for therapeutic drugs. The amino-acid sequence identity is around 74% between the RBDs in the SARS-CoV and SARS-CoV-2 S proteins, similar to the 77% identity between the whole S proteins of SARS-CoV and SARS-CoV-2. Such a high degree of sequence similarity raises the possibility that cross-reactive epitopes may exist. Although the SARS-CoV RBD polyclonal antibodies can recognize both viruses, only one out of a few published RBD monoclonal antibodies neutralizing SARS-CoV binds to SARS-CoV-2 spike RBD. Out of the 28 residues in the epitope of the S1 RBD monoclonal antibody, only 4 residues (14%) in SARS-CoV-2 S1 RBD are different from those in SARS-CoV S1 RBD. However, the S1 RBD mAb binds to SARS-CoV spike RBD (Kd = 1 nM) with a much higher affinity than to SARS-CoV-2 spike RBD (Kd = 115 nM, or 6.3 nM measured by another lab). It remains unreported that the S1 RBD neutralizing monoclonal antibody from a recovered SARS patient also behaves as a neutralizing antibody of SARS-CoV-2.
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做的比较好的，一般都是上海地区的，你可以看下基尔顿生物。
干式恒温器是采用微电脑控制和半导体制冷技术制造的一款恒温金属浴产品，仪器可配置多种模块，可广泛应用于样品的保存、各种酶的保存和反应、核酸和蛋白质的变性处理、PCR反应、电泳的预变性和血清凝固等。以下为其使作操作方法：
一、开机前检查
电源线插头已经可靠插入电源插座中，电源线接地可靠。
二、温度设置
(1)打开电源开关，所有的指示灯和数码管都亮。大约5秒后即时温度显示窗(PV)显示的数字为金属模块的即时温度，设置温度显示窗(SV)显示的数字为上一次使用的设置温度。
(2)按压(设置/SET)键一次，此时设置温度显示窗(SV)最左边数字闪烁，用上下键可更改闪烁数字到所需数值。
(3)再按压(设置/SET)键一次，闪烁数字右移一位，用上下键更改闪烁数字直至所需数值。
(4)再按压(设置/SET)键一次，闪烁数字右移一位，同样用上下键更改闪烁数字直至所需数值，完成温度的设置工作。或再次按压(设置/SET)键又从左边重新开始设置。
(5)温度设置完成后8秒，数字闪烁现象消失，表示本机系统进入运行状态，并按照当前设定的温度值运转。
三、注意事项
(1)每次使用前和使用以后，必须用棉签沾蒸馏水清洗模块的锥孔，以保证试管与锥孔壁接触充分。
(2)在设置新的温度过程中，本机系统是仍然按照上次设定的温度值运行，直至SV窗口闪烁数字停止。
(3)如果显示温度(PV)与第三方测量温度相差0.1℃以上时，联系本机保管人进行温度误差校正。
(4)在使用恒温器的过程中，禁止按压(校准/ADJ)键。
(5)如果使用在4℃恒温4小时以上的实验操作以后，必须清除模块冷凝水。方法是：拔下电源插头切断电源，然后拧开模块上两个黑色旋钮，取出模块，用软布清除各个接触面的冷凝水，然后再将模块安装复位。
第一步： 选择适用温度
了解样品最佳的实验温度，温度范围越大，相应仪器的价格也就越高，确认好了温度就可以进行下一步了。
第二步：选择试管加热类型
现有的加热类型主要分为两大类：
1 水浴：水浴最高温度在100℃
2 干浴(或称金属浴)：可选择的温度范围在100℃-250℃之间。
水浴优势：介质导热性好
水浴劣势：易污染
干式优势：洁净，使用方便
干式劣势：模块间存在极小的温差
第三步：选择模块
量一下需要使用的试管尺寸及使用数量，然后找到对应的模块。
氮吹仪就简单的选择好啦~~~
对于有更复杂功能的用户来说，只需要在提出自己的需求就好啦。
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