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StressMarq/Anti-HSP90 complex Antibody [8D3]/SMC-109B-HRP/200-µg

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StressMarq/Anti-HSP90 complex Antibody [8D3]/SMC-109B-HRP/200-µg

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StressMarq Biosciences

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SMC-109B-HRP

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Overview:

Product Name HSP90 complex Antibody

Description

Mouse Anti-Human HSP90 complex Monoclonal IgM

Species Reactivity Human, Mouse, Rat, Rabbit

Applications IP, ICC/IF

Antibody Dilution ICC/IF (1:100), IP (1:1000); optimal dilutions for assays should be determined by the user.

Host Species Mouse

Immunogen Species Human

Immunogen Ah receptor (Aryl hydrocarbon receptor)

Concentration 1 mg/ml

Conjugates

Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated

PerCP

Overview:

Peridinin-Chlorophyll-Protein Complex
Small phycobiliprotein
Isolated from red algae
Large stokes shift (195 nm)
Molecular Weight: 35 kDa

PerCP Datasheet

PerCP Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 482 nm

λ_em = 677 nm

ε_max = 1.96 x 10⁶

Laser = 488 nm

PE/ATTO 594

PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra

Optical Properties:

λ_ex = 535 nm

λ_em = 627 nm

Laser = 488 to 561 nm

FITC (Fluorescein)

Overview:

Excellent fluorescence quantum yield
High rate of photobleaching
Good solubility in water
Broad emission spectrum
pH dependent spectra
Molecular formula: C₂₀H₁₂O₅
Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 494 nm

λ_em = 520 nm

ε_max = 7.3×10⁴

Φ_f = 0.92

τ_fl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

ATTO 700

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 575 g/mol

ATTO 700 Datasheet

ATTO 700 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 700 nm

λ_em = 719 nm

ε_max = 1.25×10⁵

Φ_f = 0.25

τ_fl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

ATTO 680

Overview:

High fluorescence yield
Excellent thermal and photostability
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 631 g/mol

ATTO 680 Datasheet

ATTO 680 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 680 nm

λ_em = 700 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

ATTO 655

Overview:

High fluorescence yield
High thermal and photostability
Excellent ozone resistance
Quenched by electron donors
Very hydrophilic
Good solubility in polar solvents
Zwitterionic dye
Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 663 nm

λ_em = 684 nm

ε_max = 1.25×10⁵

Φ_f = 0.30

τ_fl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

ATTO 633

Overview:

High fluorescence yield
High thermal and photostability
Moderately hydrophilic
Good solubility in polar solvents
Stable at pH 4 – 11
Cationic dye, perchlorate salt
Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 629 nm

λ_em = 657 nm

ε_max = 1.3×10⁵

Φ_f = 0.64

τ_fl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

ATTO 594

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 1137 g/mol

ATTO 594 Datasheet

ATTO 594 Fluorophore Excitation and Emission Spectrum

Optical Properties:

λ_ex = 601 nm

λ_em = 627 nm

ε_max = 1.2×10⁵

Φ_f = 0.85

τ_fl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

ATTO 565

Overview:

High fluorescence yield
High thermal and photostability
Good solubility in polar solvents
Excellent solubility in water
Very little aggregation
Rhodamine dye derivative
Molar Mass: 611 g/mol

ATTO 565 Datasheet

ATTO 565 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 563 nm

λ_em = 592 nm

ε_max = 1.2×10⁵

Φ_f = 0.9

τ_fl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

ATTO 488

Overview:

High fluorescence yield
High photostability
Very hydrophilic
Excellent solubility in water
Very little aggregation
New dye with net charge of -1
Molar Mass: 804 g/mol

ATTO 488 Datasheet

ATTO 488 Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 501 nm

λ_em = 523 nm

ε_max = 9.0×10⁴

Φ_f = 0.80

τ_fl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

ATTO 390

Overview:

High fluorescence yield
Large Stokes-shift (89 nm)
Good photostability
Moderately hydrophilic
Good solubility in polar solvents
Coumarin derivate, uncharged
Low molar mass: 343.42 g/mol

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra

Optical Properties:

λ_ex = 390 nm

λ_em = 479 nm

ε_max = 2.4×10⁴

Φ_f = 0.90

τ_fl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

APC (Allophycocyanin)

Overview:

High quantum yield
Large phycobiliprotein
6 chromophores per molecule
Isolated from red algae
Molecular Weight: 105 kDa

APC Datasheet

APC Fluorophore Absorption and Emission Spectrum

Optical Properties:

λ_ex = 650 nm

λ_em = 660 nm

ε_max = 7.0×10⁵

Φ_f = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

Streptavidin

Properties:

Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
Molecular weight: 53 kDa
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Biotin

Properties:

Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
Molar mass: 244.31 g/mol
Formula: C₁₀H₁₆N₂O₃S
Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target
Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors
Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
High turnover rate enables rapid generation of a strong signal
44 kDa glycoprotein
Extinction coefficient: 100 (403 nm)
Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
Catalyzes the conversion of:
- Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
- Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
Molecular weight: 140 kDa
Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

R-PE (R-Phycoerythrin)

Overview:

Broad excitation spectrum
High quantum yield
Photostable
Member of the phycobiliprotein family
Isolated from red algae
Excellent solubility in water
Molecular Weight: 250 kDa

R-PE Datasheet

R-PE Fluorophore Excitation and Emission Spectra

Optical Properties:

λ_ex = 565 nm

λ_em = 575 nm

ε_max = 2.0×10⁶

Φ_f = 0.84

Brightness = 1.68 x 10³

Laser = 488 to 561 nm

Filter set = TRITC

Properties

Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide
Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Purification	PEG Purified
Clonality	Monoclonal
Clone Number	8D3
Isotype	IgM
Specificity	Detects 90kDa. Co-immunoprecipitates Hsp90 complexes, including Hsp70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2α (eIF-2α) kinase (HRI).
Cite This Product	StressMarq Biosciences Cat# SMC-109, RRID: AB_914089
Certificate of Analysis	Goat anti-mouse IgM was used to bind 25 µl of protein G-Sepharose. SMC-109 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 µl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop.

Biological Description

Alternative Names	HSP84 Antibody, HSP90 Antibody, HSP90 beta Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody, Heat shock protein HSP 90-beta Antibody, HSP 90 Antibody, Heat shock 84 kDa Antibody, HSP 84 Antibody, HSP84 Antibody, HSP90AB1 Antibody, HSP90B Antibody, HSPC2 Antibody, HSPCB Antibody
Research Areas	Cancer, Heat Shock
Cellular Localization	Cytoplasm, Melanosome
Accession Number	NP_031381.2
Gene ID	3326
Swiss Prot	P08238
Scientific Background	HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
References	1. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 2. Pearl H, et al. (2001) Adv Protein Chem 59:157-186. 3. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61. 4. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133. 5. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 6. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 7. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324–8328. 8. Perdew, G. H. (1988) JBC 263 (27): 13802-13805 9. Dalman, F. C. et al.(1989) JBC 264(33): 19815-19821 10. Uma, S. et al. (1997) JBC 272(17): 11648-11656.

Product Images

<p>Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3 (SMC-109). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody (SMC-109) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex Antibody. (C) Composite.</p>

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3 (SMC-109). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody (SMC-109) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex Antibody. (C) Composite.

<p>Immunoprecipitation analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3 (SMC-109). Tissue: reticulocyte lysate. Species: Rabbit. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody (SMC-109) at 1:1000.</p>

Immunoprecipitation analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3 (SMC-109). Tissue: reticulocyte lysate. Species: Rabbit. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody (SMC-109) at 1:1000.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 complex Monoclonal Antibody, Clone 8D3 (SMC-109). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp90 complex Monoclonal Antibody (SMC-109) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp90 complex Antibody. (C) Composite.

Product Citations (2)

Western Blot

Ganetespib (STA-9090), a Nongeldanamycin HSP90 Inhibitor, Has Potent Antitumour Activity in In Vitro and In Vivo Models of Non-Small Cell Lung Cancer.

Shimamura, T. et al. (2012) Clin Cancer Res. 18, 4973-4985.

PubMed ID: 22806877 Reactivity Human Applications: Western Blot

Other Citations

Macrocycles that inhibit the binding between heat shock protein 90 and TPR-containing proteins.

Ardi, V.C., Alexander, L.D., Johnson, V. and McAlpine S.R. (2011) ACS Chem Biol. 6 (12): 1357-1366.

PubMed ID: 21950602 Reactivity Human Applications: Immunoprecipitation

HRP (Horseradish peroxidase)

Properties:

Enzymatic activity is used to amplify weak signals and increase visibility of a target
Readily combines with hydrogen peroxide (H₂O₂) to form HRP-H₂O₂ complex which can oxidize various hydrogen donors
Catalyzes the conversion of:
- Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
- Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
- Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
High turnover rate enables rapid generation of a strong signal
44 kDa glycoprotein
Extinction coefficient: 100 (403 nm)
Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

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筛选稳定表达细胞株转染后多久药物筛选

2021-08-30

上海樊克生物科技有限公司在发布的RIPA裂解缓冲液(放射免疫沉淀)供应信息，浏览与RIPA裂解缓冲液(放射免疫沉淀)相关的产品或在搜索更多与RIPA裂解缓冲液(放射免疫沉淀)相关的内容。查看更多>

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商品咨询

药典中,纯化水酸碱度能用PH计测量吗?《中国药典》2020版讨论区...123

rainbow09282021-07-25

药典上说取本品10ml，加甲基红指示液（变色范围ph4.2-6.3，红~黄）2滴不得显红色；加溴麝香草酚蓝（变色范围ph6.0-7.6，黄~蓝）5滴不得显蓝色。
是否可以理解为纯化水得PH范围为6.3-7.6？能否直接用pH计测量？谢谢!

求大侠帮助啊,怎样确定蛋白质的酸碱性啊??? 生物科学...123

空镜2021-07-20

请问什么软件可以测蛋白质的酸碱性

一种缓冲溶液是一个共轭酸碱的混合物,那么为什么邻苯二甲酸氢钾、四硼 ...123

TWDAFRA01242017-10-03

缓冲溶液是含共轭酸碱对的，其中共轭酸与共轭碱都必须是弱酸或弱碱，这样才能组成缓冲液。因为邻苯二甲酸是弱酸，氨是弱碱，并且它们的同浓度的电离度相似，所以组成的缓冲溶液缓冲能力强。

【求助】关于流动相添加剂分析技术论坛123

快乐药师2021-07-22

我们常用的色谱柱的PH耐受大概是PH（2-9），最近摸条件，但是pH计坏了，想调一下流动相的PH，苦于PH试纸的不精确性，特此求助：

常用流动相加酸碱后PH的总结，希望大家能够提供一点自己测过的结果，谢谢先

缓冲溶液就是能抵抗外来酸碱影响,保持pH值绝对不变的溶液。 () ...123

linaqb134662017-10-02

缓冲溶液为什么能够低抗外来酸碱对ph的改变

酸碱滴定法中为什么要用缓冲溶液调节ph值123

格子控shy8032017-10-03

EDTA对金属离子的络合能力随酸度改变而不同，酸度越低，络合能力增强；酸度越高，络合能力越弱。可见控制溶液的pH在络合滴..

大家一起来讨论下哈~单抗分析:不同CEX方法测定同一个单抗,酸碱性...123

threetigers2015-03-06

如题：
两个CEX方法A和B测定同一单抗，结果碱性峰比例差不多，酸性峰比例相差约7%，相应主峰也差了7%左右。
具体来说，A方法酸性峰高，主峰低，碱性峰稍微低点；B方法酸性峰低，主峰高，碱性峰稍微高点；另外也做了CIEF，结果呢和A方法更接近。
仔细比较起来，AB两个方法的峰性和数量差不多，就不知道为什么会有这么大的差异。两个方法一个用的WCX柱-磷酸缓冲液，一个用SCX柱-MES缓冲液
大家帮我分析下：
1.两个方法哪个方法更准确，是以酸性峰高的为准还是什么？为什么？
2.这显著差异是由方法造成，具体原因是什么？柱子？
3.CIEF的结果和A方法更接近，是不是可以由此证明A方法更好或者CIEF的方法更好（因为CIEF更快更方便）？
欢迎讨论~
纠正下，A方法用的是Tosoh的柱子，B方法用的是SCX柱。TOSOH的柱子是7um的填料，10cm长。SCX是10um的填料。我本人TOSOH的阳离子柱子用的很少，这次信手用用，结果发现差异很大
那我现在就考虑，在以后方法开发过程中，除了通过流动相pH和组成、梯度、柱子选择来获得样品主峰和酸碱性的最大分离，还要关注各峰比例。因为之前比较方法好坏都只看分离度，尤其是主峰和邻近峰的分离度，获得最大分离度，自然可以做到主峰尽可能纯，但从未认真比较过各峰比例。这是一个大疏忽吧！
另外，CIEF和CEX方法原理还是有点差异的，所以分的是不同的异质体，原液放行两个方法肯定是都要做的。问题就是在早期细胞株筛选和工艺开发阶段，哪个方法才是又快又准。CIEF（iCE280)一般15分钟一个样，比CEX快多了。如果CIEF测得主峰要低于CEX结果，是不是真的完全可以取代CEX呢？CEX分离出的峰远比CIEF的多！
欢迎大家继续讨论~

如何选择缓冲溶液?_123

sweetwater2021-07-21

做试验的时候用到很多种缓冲液，Tris,triethanolamine,HEPES,还有磷酸缓冲液，不知道这些缓冲液之间有没有大的区别，选择缓冲液的依据是什么？

如果只是为了缓冲酸碱度，是不是可以用一种缓冲液来代替所有其他的？这样做试验就方便多了。谢谢指教

我是菜鸟,请教各位:强酸碱性样品可以用普通C18柱吗?谢谢了~ ...123

yandongliu2021-07-24

我的实验是考察药物不同PH值（水溶液，HCL和NaOH调PH值）下的稳定性情况等，PH从2-13。请问各位大侠：这种强酸性或强碱性水溶液样品可以直接进普通C18柱进行分析吗？
因为是考察不同PH对药物的影响，样品又不好改变其PH值，这种情况怎么办？希望有经验的高手指教。

我的流动相是甲醇-水（90：10）

谢谢赐教！

请进子版按格式发贴，自行修改，谢谢。

为什么书上说:含有缓冲混合物的缓冲液实际上是含有同离子的弱酸...123

16凌凌凌凌凌2017-10-03

就是通过微弱的中和反应控制溶液的酸碱度。

【求助】蛋白质酸碱性对离子交换色谱填料的选择蛋白质和糖学...123

ldx74226632009-08-05

求助：我目前需用离子交换法进行蛋白质的分离和纯化，但是我不知道要纯化的蛋白质的等电点，怎么样选择柱料及洗脱缓冲液呢？如果必须知道，有没有什么简便的方法测定蛋白质的等电点呢？谢谢

【求助】Whatman的DEAE52到底用不用酸碱预处理?怎么说法不一...123

langfeng2021-07-26

Whatman的网站上说DE52（货号4057-200）是预膨胀的（Preswollen），有的中文网站上说DE52是即用型。
这就是说不用酸碱预处理吗？
Whatman的网站上没有DE52最大耐受压力，请问又经验的战友应该是多少？

Whatman的网站上：
DE32DryMicrogranularDEAECellulose
SimilarperformancecharacteristicsafterprecyclingasDE52.

DE52PreswollenMicrogranularDEAECellulose
ProbablythemostwidelyusedDEAEcelluloseintheworld;usedforbiopolymerswithlowtohighnegativecharges;exhibitsexcellentresolutionwithgoodflowrates.

附件是一本图书(MethodsinMolecularMedicine,)的章节,上面说:
WhatmanDEAE52comesalreadypreswollenandonlyneedstobetransferred
totherunningbuffer50mMTE8.

lAntibodiesUsingIonExchangeChromatography.pdf(87.06k)