Description
MangoTaq™ DNA Polymerase is a formulation of Taq DNA Polymerase that offers high-yield across a wide range of DNA concentrations.
MangoTaq DNA Polymerase possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang, resulting in PCR product suitable for effective integration into TA cloning vectors.
Product Highlights
- Direct gel loading - no need for further post-PCR processing steps
- Easy visual recognition- reduces pipetting errors
- Robust performance - perfect for a wide range of PCR reactions
- Reproducible results - consistent QC ensures reliability
Product Description
MangoTaq™ DNA Polymerase offers high yield across a wide range of DNA concentrations. MangoTaq DNA Polymerase leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.
The polymerase is supplied with two different reaction buffers for greater flexibility. For high-throughput applications, MangoTaq and the colored reaction buffer make an ideal choice, since this combination enables the user to load directly on a gel in order to facilitate easy recognition.
The two reaction buffers supplied are: 5x Colored Reaction Buffer and 5x Colorless Reaction Buffer. The colored reaction buffer contains red and orange dyes, which separate during electrophoresis and provide quick reference points for monitoring the mobility of the DNA samples in the gel. The colored reaction buffer can be loaded directly onto an agarose gel for analysis without the need for separate gel-loading buffer. The presence of the dyes has no effect on routine enzymatic manipulations, although extremely rare exceptions may exist.
Since the colorless reaction buffer does not contain reference dyes, it is suitable for use when reaction products will be used directly for down-stream processes involving absorbance or fluorescent detection. The specificity and performance of MangoTaq DNA Polymerase can be further improved with the use of 3% DMSO, which is designed for GC or AT-rich DNA, "dirty" templates or sequences with a high level of secondary structure.
Applications
- High throughput applications
- Suited to a wide range of PCR assays
- Products suitable for TA cloning
- Direct loading
PCR Enzyme Guide
Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your researchPCR Selection Chart
Select the best reagent for your researchApplication Note
MangoTaq™ DNA Polymeraseebiomall.com
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加样缓冲液的主要作用是使PCR产物与其混合,使DNA沉于加样孔的底部,防止DNA跑出来.
Tris-Glycine/SDS;
MOPS/SDS;
Bis-Glycine/SDS等不同的缓冲液、预混液等。
不知道这几种缓冲液有什么异同呢？
比如如果是Invitrogen的预制胶-预混液电泳系统，每种预制胶有适配缓冲液，但是如果用其他的缓冲液似乎也可以得到不错的结果，那么是否说明电泳缓冲液只要满足了缓冲ph范围，其他的不是特别重要呢？
此外，附加提问是，PVDF膜在转膜的时候，转膜缓冲液中不需要加入甲醇，那么是加入甲醇转膜效果好还是不加好呢？
谢谢！
此外，我们常常用“电泳法”判断液体的性质，是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
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