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ReproSilDABS,4um(200x4.6mm)流动相:A:25mM醋酸钠pH=6,6/乙腈(80/20),B:乙腈/异丙醇(40/60)梯度:0min.0%B,4min.5%B,8min.20%B,15min.25%B,27min.60%B,28min.100%B,32min.100%B流速:1ml/min.检测波长:VIS436nm
温度:40℃
ReproSilFMOC-H,5um(250x4mm)流动相:A:50mM醋酸钠pH4.2/乙腈(80/20)B=50mM醋酸钠pH4.2/乙腈(20/80)梯度:0min.0B,35min.40%B,45min.70%B,50min.100%B,55min.100%B.
检测器:Fluor.:Ex.:263nm,Em.:310nm流速:1ml/min.进样量:10ul温度:40℃
Column:ReproSilOPA,3um(150x4.6mm)
AnalysisofAminoAcidsbypre-columnDerivatisationwith通过OPA/3-MPA衍生化分析氨基酸梯度:A:25mM磷酸钠缓冲盐(pH=7,2)/THF(995/5)B:25mM磷酸钠缓冲盐(pH=7,2)甲醇/乙腈(50/35/15)梯度:0-3min.0%B,bei20min.35%B,25min.50%B,36min.60%B,40min.70%B,43-45min.100%B.
流速:1ml/min.Fluor.-检测器:Ex330nmEM.450nm