eIF4E Detection Kit
GloboZymes eIF4E Detection Kit includes 100 μl of rabbit Anti-eIF4E polyclonal antibody and an aliquot of highly purified recombinant human eIF4E. The eIF4E preparations are provided in SDS sample buffer ready for use in five applications as positive control in Western blots. The antibody is also effective for immunoprecipitation.
Synonyms: Eukaryotic Protein Synthesis Initiation Factor 4E Western Blot Detection Kit; eIF-4E Western Blot Detection Kit
Background: The mRNA Cap-binding protein synthesis initiation factor 4E (eIF4E) participates in an early rate limiting step in protein synthesis. Over-expression of eIF4E leads to cell transformation and tumorigenesis and occurs in a number of cancer cells. eIF4E occurs as an inactive complex with the 4EBP binding proteins in quiescent cells. However, in response to insulin and other extracellular stimuli, eIF4E dissociates from 4EBP and is recruited to the active eIF4F complex. In addition to eIF4E, the eIF4F complex contains eIF4A and eIF4G. In cells exposed to insulin and other growth factors, eIF4E is phosphorylated at Ser209. The affinity of eIF4E to capped mRNA is enhanced by this phosphorylation, which is catalyzed by the Mitogen-Activated Protein Kinase (MAPK) Interacting Kinases (Mnk’s). It is also catalyzed by an insulin-stimulated protein kinase termed cPK which also phosphorylates Thr210 on the initiation factor.
Figure: Western blot of purified preparation of eIF4E with Anti-eF4E.
References: J Biol Chem 270, 14824-14828; J Biol Chem 270, 14597-14603; Biochime 76, 822-830; J Biol Chem 271, 11831-11837; J Biol Chem 270, 21684-21688
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3-硝基邻苯二甲酸可作为鲁米诺的合成原料。3-硝基邻苯二甲酸与肼在高沸点溶剂（如二甘醇）中发生缩合反应，失去一分子水，生成3-硝基邻苯二甲酰肼。然后以保险粉还原3-硝基邻苯二甲酰肼中的硝基，得到3-氨基邻苯二甲酰肼，即是鲁米诺。
我们常说的鲁米诺试剂是鲁米诺与过氧化氢（双氧水的主要成分）的混合物，主要用于现代刑侦的的血液检测。鲁米诺与氢氧化物反应时生成了一个双负离子（Dianion），它可被过氧化氢分解出的氧气氧化，产物为一个有机过氧化物。该过氧化物很不稳定，立即分解出氮气（鲁米诺被有机氧化剂如二甲基亚砜氧化后不是生成氮气，而是生成含氮有机物），生成激发态的3-氨基邻苯二甲酸。激发态至基态转化中，释放的能量以光子的形式存在，波长位于可见光的蓝光部分。鲁米诺只有用氧化剂处理过才会发光。通常使用双氧水和一种氢氧化物碱的混合水溶液作为激发剂。在铁化合物催化下，双氧水分解为氧气和水：2H2O2→O2+2H2O实验室中常以铁氰化钾作为催化剂铁的来源，而法医学上的催化剂则恰好是血红蛋白中的铁。很多生物系统中的酶也可催化过氧化氢的分解反应。鲁米诺试剂使用识别血液的试剂，血迹即使被擦拭，血液中的血红素还是会残留下来，当鲁米诺试剂喷在血红素上，会与活性氧产生氧化作用，释放出蓝紫色荧光。被成为鲁米诺反应。是鉴定血液用的有机物质。
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你是说用另外的方法来验证结果吗？
钡离子焰色反应为黄绿色
钙离子燃烧砖红色
铷离子焰色反应为紫色
铜离子焰色反应为绿色
钠离子焰色反应为黄色
钾离子焰色反应为浅紫色
锶离子焰色反应为洋红色
经常使用这些元素的硝酸盐作为焰火发色剂。
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