MakingAntibody ·ProductionofPolyclonalAntibodyinRabbit(WalterSteffen)Providesdetailedprotocolforimmunizatioin,bleedingprocedure,obtainingserum,precipitationofIgG. ·GST-fusionproteininjectionintorabbits(IVANJ.DELGADOORLIC) ·HowDoImakeanAntibodyagainstaPeptides(Pierce) ·Afastandinexpensivemethodforraisinghigh-specificitypolyclonalantibodiesinmice(TechnicalTipsOnline) ·GuidetoAntipeptideAntibody(Perkin-Elmer)GeneralInformationaboutthedesignandsynthesisofpeptidesfortheproductionofanti-peptideantibodies,aswellasanoverviewofthestepsinvolvedintheproductionofantibodiesimmunization,collectionofantisera,analysisofantisera,andantibodypurification.Protocolsforanumberoftheseproceduresareprovided. oDesigningAntigens oSynthesizingPeptides oConjugationofPeptidestoProteinCarriers oImmunizations oAnalysisofAntisera:Immunoassays oPurifyingAntibodies Others ·Tipsandhintsforthestorageofantibodies(SynapticSystems)