ORDERINGINFORMATION
CatalogNo.:43026
Format:100ulat1.0mg/mlinPBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol.Affinity-purifiedonphosphopeptide;non-phosphopeptide-reactiveantibodieswereremovedbychromatographyonnon-phosphorylatedpeptide.
BACKGROUND
c-Junincombinationwithc-Fos,formstheAP-1earlyresponsetranscriptionfactor.ItwasfirstidentifiedastheFos-bindingproteinp39andonlylaterrediscoveredastheproductofthec-jungene.ItisactivatedthroughdoublephosphorylationbytheJNKpathwaybuthasalsoaphosphorylation-independentfunction
SPECIFICATIONSUMMARY
Antigen:Peptidesequencethatincludesphosphorylationsiteofserine79(G-A-S(p)-L-K)derivedfromhumanc-JunandconjugatedtoKLH.
Accessionno.:P05412,NP_002219.1
HostSpecies:Rabbit
Specificity:Thisantibodydetectsendogenoushuman,mouse,andratc-Junonlywhenphosphorylatedatserine79.
APPLICATION
Immunohistochemistry:useatdilutionof1:50-1:100.
Immunofluorescence:useatdilutionof1:100-1:200.
Detectionofc-Jun(phospho-Ser79)inparaffin-embeddedhumanbreastcarcinomatissue.
Detectionofc-Jun(phospho-Ser79)inmethanol-fixedHeLacells.
Thesearerecommendedworkingdilutions.Endusershoulddetermineoptimaldilutionsfortheirapplications.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oC.Canbestoredat4oCforshort-termuse.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticapplications.
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二抗
抗体组合
免疫组化
免疫沉淀
ELISA
ELISPOT
流式
同型对照
酪胺信号放大
免疫对照/血清/阻断/封闭
Western Blot免疫印迹
免疫缓冲剂/固定/透化
细胞/组织裂解物
细胞核裂解物
细胞裂解物
组织抽提物
转染细胞裂解物
CBA蛋白定量
链霉亲和素
抗体纯化
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像;
同形像统计图
请问:同型对照应该怎么设?
不买专门的IgG2Bisotypecontrol,而用其他的抗体,
比如mouseanti-humanCD117,Igclass也是IgG2B,可以吗?
暂无品牌问答