About InVivoMAb anti-mouse/human/rat v-H-Ras
The Y13-238 monoclonal antibody reacts with human, mouse, and rat v-H-ras (within amino acids 120-138) and does not react with v-K-ras. v-H-ras binds GTP/GDP and has intrinsic GTPase activity. Ras proteins alternate between an inactive form bound to GDP and an active form bound to GTP, activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP). Under normal conditions, Ras family members influence cell growth and differentiation events. Mutations in the Ras family of proto-oncogenes are very common, being found in 20% to 30% of all human tumors. Ras point mutations are the single most common abnormality of human proto-oncogenes. The Y13-238 antibody does not neutralize ras GTPase binding and hydrolysis in vivo or in vitro.
InVivoMAb anti-mouse/human/rat v-H-Ras Specifications
Isotype | Rat IgG2a |
Recommended Isotype Control(s) | InVivoMAb rat IgG2a isotype control, anti-trinitrophenol(BE0089) |
Recommended InVivoPure Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer(IP0070) |
Immunogen | Harvey murine sarcoma virus infected NRK cells |
Reported Applications | Immunoprecipitation |
Endotoxin |
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Purity |
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Formulation |
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Sterility | 0.2 μM filtered |
Production | Purified from tissue culture supernatant in an animal free facility |
Purification | Protein G |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
RRID | AB_10951512 |
Molecular Weight | 150 kDa |
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二抗
抗体组合
免疫组化
免疫沉淀
ELISA
ELISPOT
流式
同型对照
酪胺信号放大
免疫对照/血清/阻断/封闭
Western Blot免疫印迹
免疫缓冲剂/固定/透化
细胞/组织裂解物
细胞核裂解物
细胞裂解物
组织抽提物
转染细胞裂解物
CBA蛋白定量
链霉亲和素
抗体纯化
英 ['aisəutaip]
美 ['aisəutaip]
n. 同形像;
同形像统计图
阴性对照(Isotype Control):非特异荧光的强弱取决于抗体浓度、单克隆荧光抗体特异性和纯度,应与试验管抗体相对应。在多色分析时,同型对照应与其它抗体同时使用,以避免补偿造成的误差
血小板体外活化试验:使用正常人活化标本作为阳性质控;使用正常人未活化标本作为阴性质控
血小板自身抗体检测:使用含有已知血小板抗体的血清与血小板孵育,作为阳性质控;使用不含血小板抗体的血清与血小板孵育,作为阴性质控
血小板表面抗原缺失:如巨血小板症血小板表面CD42a/CD42b缺失,血小板无力症血小板表面gpIIb/IIIa,即CD41/CD61缺失或异常。使用正常人标本做阳性对照,抗体的同型对照做阴性对照
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