Specifications
UM413MUbiTest:K63Linkage-SpecificMagnetic-TUBEElutionKit
Thiskitallowsyoutodetectwhetheryourtargetproteinisubiquitylatedanddeterminethelinkagetypeoftheubiquitinchain.
Determiningthelinkageofpolyubiquitinontargetproteinsischallenging.ThetrADItionalmethodsareeitherthroughMassSpectrometrywhichiscumbersomeorimmunoblotwith linkagespecific antibodies,whichareunreliable.LifeSensorshasdevelopedamoredefinitivemethodfordemonstratingtheubiquitylationlinkageofaprotein,whichistocoupleimmunoprecipitationofpolyubiquitylatedproteinwithdigestionbya linkagespecificdeubiquitylase priortoimmunoblotanalysis. Anincreasedsignalfortheunmodifiedsubstrateoradecreasedsignalof polyubiquitylated substrateat high molecularweightafterK48/K63specificDUBtreatmentisaclearindicationthattheproteinwasK48/K63ubiquitylated. WehavebuiltthiskitaroundtheuseofMagneticTandemUbiquitinBindingEntities(TUBEs)whichbindtoallubiquitinchainlinkages.Magnetic-TUBEsareTUBEmoietiesdirectlycoupledtomagneticbeads,fortheidentificationandcharacterizationofpolyubiquitinatedproteinsbywesternblottingand/ordownstreamproteomicstudies. Magnetic-TUBEsfacilitateconvenient“one-step”pull-downofpolyubiquitinatedproteins.
ThiskitincludesthefollowingDUBs:
- ALys63linkagespecificDUB–35 µg,10 µM
- AbroadspectrumDUB–25 µg,10 µM
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因此所有羊抗兔可以结合兔子的同种异型抗体,也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属:兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应:鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用:用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验
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