H3K4me1 Rabbit Polyconal Antibody
Reactivity: H M Rat Other (Wide Range)
Tested applications: WB IHC IF
Recommended Dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Detected band (by western)MW: 15kDa
Immunogen: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3
Storage Buffer: Store at: -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Concentration: 1mg/ml
Synonym: HIST1H3J; H3/j ;H3FJ ; Histone H3.1; Histone H3/a; Histone H3/b;Histone H3/c; Histone H3/d; Histone H3/f;Histone H3/h ; Histone H3/I;HistoneH3/j ;Histone H3/k; Histone H3/l;HIST3H3;
H3K4 methylation is one of the gene activation markers and one of the hottest epigenetic targets in recent studies. H3K4me1, H3K4me2 and H3K4me3 have a commonality for gene activation, although their subsets play distinct roles in modulating chromatin function (4). Among which, H3K4me3 occupies as many as 75% of all human gene promoters in several cell types (e.g., ES cells), playing important role in mammalian gene expression (1). H3K4me2 decorates genomic regions independently of H3K4me3, although most of it overlaps with H3K4me3 near the transcription start sites (2). Monomethylated H3K4 (H3K4me1) also co-occupies regions near the start sites with H3K4me3. Apart from the transcription start site, H3K4me1, together with H2K27 acetylation, specifies enhancer regions (3).
In recent studies, H3K4 has been demonstrated to be related stem cell self-renewal and differentiation (4).
H3K4 methylation statuses can be detected by our H3K4 methylation antibody series.
Please refer PDF file for Western, Immunofluorescence analysis,Chromatin immunoprecipitation analysis or other QC data.
H3K4_Monomethylated_Polyclonal_Antibody.pdf
SDS_H3K4me1_Polyclonal_Antibody.pdff
References:
- Pan G, TianS, Nie J, Thomson JA: Whole-genome analysis of histone H3 lysine 4 and Lysine 27. Cell Stem Cell 2007, 1:299-312.
- 2. Bernstein BE, Kamal M, Lindblad-Toh K, Gingeras TR, et al: Genomic maps and comparative analysis of histone modifications in human and mouse. Cell 2005, 120: 169-181.
- 3. Visel A, Blow MJ, Liz, Zhang T, Wright C, Chen F, et al: CHIP-seq accurately predicts tissue-specific activity of enhancers.Nature 2009, 457: 854-858.
- 4. Bingnan Gu and Min Gyu Lee: Hsitone H3 lysine 4 methyltransferases and demethylases in self-renewal and differentiation of stem cells.Cell & Biosciences 2013, 3: 39.
Product is for research use only. Not for human use.
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因此所有羊抗兔可以结合兔子的同种异型抗体,也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属:兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应:鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用:用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验
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