Specifications
TUBEs: ARevolutioninPolyubiquitinIsolationandCharacterization
TheuseofTandemUbiquitinBindingEntities(TUBEs)isemergingasanindispensablestrategyforubiquitinresearch.ThefirstgenerationoftheseTUBEsbindK48-andK63-linkedtetraUbchainswithsingledigitnanomolarKds,~100to1000-foldmoretightlythanmonomericUBAs.TUBEsalsoprotectproteinsfromDUBsandtheproteasome,evenintheabsenceofinhibitorsnormallyrequiredtoblocksuchactivity.ThisallowsefficientisolationofnativepolyUbchainsandattachedproteinsfromcelllines,tissues,andorgansunderconditionsthatarelesslikelytoaltercellphysiologythanthoselistedabove.TUBE1andTUBE2haverecentlybeendemonstratedtoenrichforallpolyUbchainlinkagetypes,withoutdiscrimination,makingthesereagentsappropriateevenifthelinkagetypeisnotknownfortheproteinofinterest.
ThenextgenerationofTUBEs: LinkageSpecificIsolationofPolyUbChains
K48TUBEHFwasdevelopedtoshowenhancedselectivityforK48-linkedpolyubiquitinchains(~20nM)overallotherlinkages(>2µM).ItcanbeusedaloneorinconjunctionwithourotherTUBEproducts,especiallyK63TUBEandM1(linear)TUBEtoinvestigatepolyubiquitinchainlinkageinyoursubstrateprotein.
Note:ThenewK48TUBEHFhasacomparableaffinityasourexistingK63TUBE.BothofthembindtotheircognateUb4swithaKdof~10-20nM.
Magnetic-TUBEsareTUBEmoietiesdirectlycoupledtomagneticbeads,fortheidentificationandcharacterizationofpolyubiquitinatedproteinsbywesternblottingand/ordownstreamproteomicstudies.Magnetic-TUBEsfacilitateconvenient“one-step”pull-downofpolyubiquitinatedproteins.
Info
K48TUBEHF,MagneticSize:1mlBuffer:PBS,pH7.2,20%ethanolStorage:Stableforupto6monthsat4oC.Donotcentrifugeathighspeeds(>3000g),dryorfreezethebeads.
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因此所有羊抗兔可以结合兔子的同种异型抗体,也就是说只要是兔子产生的抗体,羊抗兔(也叫抗抗体或者2抗)都可与之结合。
在试验中,二抗经常被做了手脚,打上了标记,比如辣根过氧化物酶,经常被用在ELISA,WESTERN等经典的生物学试验中。
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体种属:兔抗单克隆抗体鼠抗单克隆抗体抗体浓度1mg/ml
抗体相关标记:HRP,Biotin,Gold,RBITC,AP,FITC,Cy3,Cy5,Cy5.5,Cy7,PE,PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7,APC,Alexa Fluor 350,Alexa Fluor 488,Alexa Fluor 555,
Alexa Fluor 647
抗体交叉反应:鼠鼠鸡 狗猪羊牛兔等......
Anti-HIF-1 Alpha抗体,缺氧诱导1α 抗体HIF-1α抗体应用:用于做石蜡切片免疫组化冰冻切片免疫组化ElisaWB免疫荧光等实验
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