The3G3antibodyreactswithmouseFoxp3,a50-55kDatranscriptionfactorwhichisacentralregulatorofTcellactivityandiscriticalforthedevelopmentandfunctionofregulatoryTcells(Tregs).Foxp3isexpressedatconstitutivelyhighlevelsinTregcells,whicharefurtheridentifiedasbeingCD4+CD25+.InrestingconventionalTcells(CD4+CD25-)Foxp3expressionisrestricted,anduponTCRactivationisexpressedonlytransientlyandinasmallproportionofcells.However,thegrowthfactorTGF-betahasbeenshowntoinduceexpressionofFoxp3innaïveTcells,drivingtheirdevelopmentintoFoxp3+Tregs,whicharecalled“induced”or“adaptive”Tregs.Thesecellsarephenotypicallysimilartoso-called“natural”Tregs(CD4+CD25highFoxp3+)whichoriginateinthethymusandcomprisethemajorityofTregcells.Tregsarecriticalformaintainingperipheraltoleranceandareimplicatedinthedevelopmentofautoimmunity.
ItisimportanttoreviewtheliteratureinchoosinganantibodyfortheFoxp3antigeninflowcytometry,asthepotentialforhighbackgroundornon-specificstainingmaybeobserved.The3G3antibodymaybeusedforintracellulardetectionofFoxp3incellsfrommouseandRhesusmacaque.
| Name | APCAnti-MouseFoxp3(3G3) |
|---|---|
| Cat.No. | 20-5773 |
| AlternativeNames | ForkheadBoxP3,Scurfin |
| GeneID | 50943/20371 |
| Clone | 3G3 |
| Isotype | MouseIgG1,kappa |
| Reactivity | Mouse |
| CrossReactivity | Rhesusmacaque |
| Format | APC |
| Applications | FlowCytometry |
| Citations* | RamosRN,OliveiraCE,GasparotoTH,etal.2012.Carcinogenesis.33:902-909.(Flowcytometry) KleinM,VaethM,ScheelT,GrabbeS,BaumgrassR,Berberich-SiebeltF,BoppT,SchmittE,andBeckerC.2012.J.Immunol.188:1091-1097.(Flowcytometry) AnsariAA,ReimannKA,MayneAE,TakahashiY,StephensonST,WangR,WangX,LiJ,PriceAA,LittleDM,ZaidiM,LylesR,andVillingerF.2011.J.Immunol.186:1044-1059.(Flowcytometry–Rhesusmacaque) NagarM,VernitskyH,CohenY,DominissiniD,BerkunY,RechaviG,AmariglioN,andGoldsteinI.2008.Int.Immunol.20:1041-1055.(Flowcytometry) HombachAA,KoflerD,HombachA,RapplG,andAbkenH.2007.J.Immunol.179:7924-7931.(Flowcytometry). GavinMA,TorgersonTR,HoustonE,deRoosP,HoWY,Stray-PedersenA,OcheltreeEL,GreenbergPD,OchsHD,andRudenskyAY.2006.ProcNatlAcadSciUSA.103(17):6659-6664.(Flowcytometry) |
