- SynonymHER4,ErbB4,MGC138404,p180erbB4
- SourceHuman ErbB4, His Tag (ER4-H5221) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Pro 651 (Accession # NP_005226.1).Predicted N-terminus: Gln 26Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.7 kDa. The protein migrates as 95-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehaloseis added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human ErbB4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human ErbB4, His Tag (Cat. No. ER4-H5221) at 2 μg/mL (100 μL/well) can bind Human NRG1 Beta 1, Fc Tag (Cat. No. NR1-H5268) with a linear range of 0.5-8 ng/mL (QC tested).
- BackgroundReceptor tyrosine-protein kinase erbB-4 (ErbB4), also known as Her4, is a single-pass type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ERBB4 contains multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins-2 and -3, heparin-binding EGF-like growth factor and betacellulin. Ligand binding induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer. ERBB4 has been shown to interact with: DLG4, NRG1, STAT5A, and YAP1. Mutations in this gene have been associated with cancer. Other single-nucleotide polymorphisms and a risk haplotype have been linked to schizophrenia.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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试题分析:酶是一种生物催化剂,在催化反应中本身不发生变化;此非酶的特性;B项体现了酶的作用条件较温和的特性;C项体现了酶的专一性;D项体现了酶的高效性;故选A。
考点:本题考查的是酶的特性。
点评:对于此类试题,学生应掌握酶的特性。
BAS-ELISA是在常规ELISA原理的基础上,结合生物素(B)与亲和素(A)间的高度放大作用,而建立的一种检测系统。生物素很易与蛋白质(如抗体等)以共价键结合。这样,结合了酶的亲和素分子与结合有特异性抗体的生物素分子产生反应,既起到了多级放大作用,又由于酶在遇到相应底物时的催化作用而呈色,达到检测未知抗原(或抗体)分子的目的。
辣根过氧化物酶(Horseradish Peroxidase,HRP)比活性高,稳定,分子量小,纯酶容易制备,所以最常用。HRP广泛分布于植物界,辣根中含量高,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%。HRP由多个同功酶组成,分子量为40,000,等电点为PH3~9,酶催化的最适PH因供氢体不同而稍有差异,但多在PH5左右。酶溶于水和58%以下饱和度硫酸铵溶液。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm,一般以OD403nm /OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。高纯度的酶RZ值应在3.0左右(最高可达3.4)。RZ值越小,非酶蛋白就越多。 1) RZ>3 活性 >250u/mg, 主要应用于免疫学,是高纯度的过氧化酶。采用特殊色谱纯化技术以除去会影响免疫学反应的同工酶B .
2) RZ>2 活性 >180u/mg ,主要应用于临床化学,我们的客户也有将这个规格的产品应用于免疫学研究的。此时,一个标准化的分析方法就变得尤为重要。
3) RZ>1 活性 >100u/mg,主要应用于血糖试纸和尿液分析试纸。
4) RZ>0.6 活性 >60u/mg ,主要应用于尿液分析试纸。向左转|向右转
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