Source | human 293 cells | M.W. | ~180 kDa | CAS No. | |
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Structural Info | |||||
Formulation | Lyophilized in a sterile solution of PBS. | ||||
Reconstitution | Before reconstitution, werecommend a brief spin to drive down any material dislodged from the bottom ofthe tube. The lyophilized peptide shouldbe reconstituted in sterile water. | ||||
Stability | The lyophilized protein isstable for at least 1 year if stored at -80°C. Reconstituted protein is stable for at least 1 month at 4 °C, but shouldbe stored in aliquots at -80°C for longer term. Avoid repeated freeze andthaw. | ||||
Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
Biological Activity | Determined by a Notch pathway specific reporter gene assay.EC50s are in the range of 200 – 1000 ng/mL. | ||||
Country of Origin | USA |
The Notch pathwayis an important pathway in stem cell functions.Jagged-1, a ligand for Notch receptors, is a 180kDa type 1 membraneglycoprotein of the Delta/Serrate/Lag2 (DSL) family.StemRD’s Jagged-1 Fcfusion protein is expressed in human 293 cells as the 1046aa N-terminalextracellular domain of Jagged-1 fused to the Fc domain of human IgG1. This fusion stabilizes Jagged-1 in vitro andin vivo without significant compromise in its biological activity.
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SP法是生物素-卵白素系统:生物素为一种小分子维生素,卵白素又称为抗生物素,SP法的染色步骤为一抗~生物素化二抗~ABC复合物~DAB显色,其中ABC复合物是由一分子抗生物素蛋白与三分子结合有HRP的生物素组合成的复合物。
PV法属于非生物素系统免疫组化方法,应该是称为二步法,是以高分子的葡聚糖为载体将多个二抗分子和酶结合在一起,代替了传统的二抗和三抗,是检测变得简单而且增加了敏感性。
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