The Carlavirus Group PCR primers offer a sensitive diagnostic method to detect members of the Carlavirus genus of the Betaflexiviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Carlavirus genus.This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.The Carlavirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd.Purchase of this product does not include the license under these patents to use the PCR process.This product is recommended for use in conjunction with an authorized thermal cycler.
User Guide
This test reacts with a variety of viruses from the Carlavirus genus. The list below represents Carlaviruses that have been experimentally detected with our Carlavirus PCR and confirmed by sequencing. If you have confirmed detection of a Carlavirus not on this list, please contact us. For viruses predicted to be detected, click here.
Confirmed Detections | |
---|---|
Blueberry scorch virus (BlScV) | Carnation latent virus (CLV) |
Chrysanthemum virus B (CVB) | Coleus vein necrosis virus (CVNV) |
Cowpea mild mottle virus (CPMMV) | Garlic common latent virus (GarCLV) |
Helleborus mosaic virus (HeMV) | Hellebore net necrosis virus (HeNNV) |
Hop latent virus (HpLV) | Hydrangea chlorotic mottle virus (HdCMV) |
Kalanchoe latent virus (KLV) | Ligustrum necrotic ringspot virus (LNRSV) |
Lily symptomless virus (LSV) | Narcissus symptomless virus (NeLV-NSV) |
Nerine latent virus (NeLV) | Pea streak virus (PeSV) |
Phlox virus M (PhlVM) | Potato latent virus (PotLV) |
Potato virus M (PVM) | Potato virus S (PVS) |
Red clover vein mosaic virus (RCVMV) | Shallot latent virus (SLV) |
This test reacts with a variety of viruses from the Carlavirus genus. The list below represents Carlaviruses that have not been experimentally detected, but based on in silico analysis, will likely be detected with our Carlavirus PCR. If you have samples of these pathogens below, please contact us.
Predicted Detections | |
---|---|
Aconitum latent virus (AcLV) | American hop latent virus (AHLV) |
Butterbur mosaic virus (ButMV) | Caper latent virus (CapLV) |
Cucumber vein-clearing virus (CuVCV) | Daphne virus S (DVS) |
Elderberry symptomless virus (ElSLV) | Hippeastrum latent virus (HLV) |
Hop mosaic virus (HpMV) | Melon yellowing-associated virus (MYaV) |
Mirabilis jalapa mottle virus (MjMV) | Narcissus common latent virus (NCLV) |
Passiflora latent virus (PLV) | Phlox virus B (PhlVB) |
Phlox virus S (PhlVS) | Poplar mosaic virus (PopMV) |
Potato virus P (PVP) | Sint-Jan"s onion latent virus (SJOLV) |
Sweet potato C6 virus (SC6V) | Sweet potato chlorotic fleck virus (SPCFV) |
Verbena latent virus (VeLV) | Potato virus H (PVH)* |
Twisted-stalk leaf streak virus (TSLSV)* |
* Tentative Carlaviruses
Includes:
- Lyophilized forward and reverse primers
- Sterile PCR quality water
- User Guide
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SP法是生物素-卵白素系统:生物素为一种小分子维生素,卵白素又称为抗生物素,SP法的染色步骤为一抗~生物素化二抗~ABC复合物~DAB显色,其中ABC复合物是由一分子抗生物素蛋白与三分子结合有HRP的生物素组合成的复合物。
PV法属于非生物素系统免疫组化方法,应该是称为二步法,是以高分子的葡聚糖为载体将多个二抗分子和酶结合在一起,代替了传统的二抗和三抗,是检测变得简单而且增加了敏感性。
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