- SynonymIL-1 R6,IL-1 Rrp2,IL1RL2,IL-1RL2,IL1Rrp2,IL-1Rrp2,IL1RRP2,IL-36R
- SourceHuman IL-1 Rrp2 (C154S, C262S), His Tag (IL2-H52H4) is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg 335 (Accession # Q9HB29-1(C154S, C262S)).Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.9 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human IL-1 Rrp2 (C154S, C262S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-1 Rrp2 (C154S, C262S), His Tag(Cat. No. IL2-H52H4) was more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.
Immobilized Human IL-1 Rrp2 (C154S, C262S), His Tag (Cat. No. IL2-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-Human IL-1 Rrp2 MAb, Human IgG with a linear range of 0.2-3 ng/mL (Routinely tested).
- BackgroundThe Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9).All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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我想在体外培养的细胞中,加入PD-L1重组蛋白,激活PD-L1:PD-1通路,但我没能查到相关的文献,不清楚PD-L1重组蛋白的用量。请问各位有相关的经验吗?或者阅读过相关的文献?
定量:考马斯亮蓝显色或紫外分光光度计,就是光谱学的方法。
人类医学发展到今天,对某些疑难疾病还是不能彻底根治,如遗传疾病,器官坏死,和糖尿病等。这些疾病仅靠药物治疗只能减缓症状,而器官移植又受捐赠器官有限和免疫排斥等因素的限制而不能推广。干细胞是唯一有可能攻克这些疾病的治疗手段。但长期以来,获取多能干细胞(pluripotentstemcell)的主要来源为人的胚胎。这引起道德和宗教的争议,进而在美国等国家受到法律限制。另外,用异己的胚胎干细胞发展的治疗手段将来还是会遇到免疫排斥的问题。
2006年,日本科学家ShinyaYamanaka领导的实验室第一次证明,通过以反转录病毒为载体转基因表达四个转录因子,可以将小鼠或人的体细胞转变为与胚胎干细胞拥有相似分化和繁殖能力的细胞,命名为诱导性干细胞(iPS)。这一成果具有划时代的意义:1)干细胞的产生可以不再需要破坏胚胎,避免了道德,宗教和法律上的限制;2)不同疾病的诱导性干细胞可衍生出不同类型的疾病模型,为基础研究和药物筛选提供了强大的武器;3)理论上,各种疑难疾病可以通过病人自己的体细胞转变为干细胞,再分化为各种类型的细胞,组织,甚至器官,经过或不经过体外加工后,放回病人体内,治愈疾病。
在本论文中,研究人员成功地用四个转录因子的蛋白完成了由体细胞到诱导性干细胞的转变过程。自始至终,细胞的遗传信息没有受到任何影响。蛋白诱导性干细胞的重大意义包括:1)安全性:转化过程没有使用病毒,没有使用基因,没有任何改变细胞遗传信息的风险;2)普及性:蛋白诱导方法远比基因诱导方法简单易行。这样一来,iPS技术不再被几个资深实验室所垄断。大部分实验室都可以重复蛋白方法而获得iPS。也就是说,蛋白诱导方法大大降低了iPS领域的"门坎"。3)可行性:由于该方法的简单易行,重复性强,它可以被扩大化,产业化,进而被商业化。蛋白诱导干细胞方法将大大降低利用干细胞对病人"量身定做"的治疗手段的成本,使得这一商业模式成为可能。
如果说,ShinyaYamanaka博士的成果第一次使人类看到了一个梦想,那么这篇论文的发表标志着我们从梦想向现实跨出了关键的一大步。
CellStemCell,23April2009doi:10.1016/j.stem.2009.04.005
GenerationofInducedPluripotentStemCellsUsingRecombinantProteins
HongyanZhou1,ShiliWu4,7,JinYoungJoo5,7,SaiyongZhu1,DongWookHan5,TongxiangLin1,SuniaTrauger2,3,GeofferyBien4,SusanYao4,YongZhu4,GarySiuzdak2,3,HansR.Sch?ler5,LingxunDuan6andShengDing1,,
1DepartmentofChemistry,TheScrippsResearchInstitute,10550NorthTorreyPinesRoad,LaJolla,CA92037,USA
2DepartmentofMolecularBIOLOGy,TheScrippsResearchInstitute,10550NorthTorreyPinesRoad,LaJolla,CA92037,USA
3CenterforMassSpectrometry,TheScrippsResearchInstitute,10550NorthTorreyPinesRoad,LaJolla,CA92037,USA
4ProteomTech,Inc.,3505CADIllacAvenue,SuiteF7,CostaMesa,CA92626,USA
5DepartmentofCellandDevelopmentalBiology,MaxPlanckInstituteforMolecularBiomedicine,R?ntgenstrasse20,Münster48149,Germany
6LD***aInc.,SandownWay,SanDiego,CA92130,USA
7Theseauthorscontributedequallytothiswork
Groundbreakingworkdemonstratedthatectopicexpressionoffourtranscriptionfactors,Oct4,Klf4,Sox2,andc-Myc,couldreprogrammurinesomaticcellstoinducedpluripotentstemcells(iPSCs)(TakahashiandYamanaka,2006),andhumaniPSCsweresubsequentlygeneratedusingsimilargeneticmanipulation(Takahashietal.,2007,Yuetal.,2007).Toaddressthesafetyissuesarosefromharboringintegratedexogenoussequencesinthetargetcellgenome,anumberofmodifiedgeneticmethodshavebeendevelopedandproducediPSCswithpotentiallyreducedrisks(fordiscussion,seeYamanaka,2009,andreferencestherein).However,allofthemethodsdevelopedtodatestillinvolvetheuseofgeneticmaterialsandthusthepotentialforunexpectedgeneticmodificationsbytheexogenoussequencesinthetargetcells.Herewereportgenerationofprotein-inducedpluripotentstemcells(piPSCs)frommurineembryonicfibroblastsusingrecombinantcell-penetratingreprogrammingproteins.WedemonstratedthatsuchpiPSCscanlong-termself-renewandarepluripotentinvitroandinvivo.
国内或者国外进口皆可!?看来你不缺钱啊!
这个问题问的过于笼统!
首先,蛋白表达与纯化包括很多种类型,比如原核蛋白表达,哺乳动物蛋白表达,酵母蛋白表达以及昆虫蛋白表达等等,而现在生物实验中常说的蛋白表达纯化通常是指利用大肠杆菌表达系统的原核蛋白表达,这种表达方式比较简单,普遍都可以做。但是如果是指很多种蛋白表达系统的话,可以做的单位就比较少了。
另外,蛋白的表达成功与否还需要取决于蛋白的性质,所以前期一定要问清楚!
开个贴,做好准备,已经有战友开始采用CTD格式写申报资料,大家多交流。
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