AQUApure Tri-Ub Chains (K33-linked) Protein, CF Summary
Ub-AQUA analysis:
K33: 97.23%
K29: 1.83%
K48: 0.31%
K63: 0.26%
K6 : 0.21%
All other linkages ≤ 0.13%
Ubiquitin chains vary in length, linkage, and function. K33-linked Tri-Ubiquitin Chains (Ub3) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °c="" for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">40>
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-105
Formulation | 1 mg/ml (39 μM) in sterile, deionized water. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Tri-Ubiquitin
Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB"s") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF"s and/or other Ubiquitin-sensing elements.
K33-linked Tri-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates. The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.
- Kirkpatrick D.S., et al. (2006) Nat Cell Biol. 8(7): 700-10
- Michel, M.A. et al. (2015) Mol Cell. 58(1): 95-109
- Ordureau, A., et al. (2014) Mol. Cell 56(3): 360–375
- Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA 112(21): 6637–6642
- Phu L., et al. (2011) Mol Cell Proteomics 10(5): M110.003756
FAQs
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家用的菜刀也叫切片机,工业用的医院切组织用的也叫切片机。
不同的切片机切片的方法也不同,比如试验中要处理细胞或者组织,从而方便用显微镜进行观察实验。用于光学显微镜的有旋转式和滑走式切片机
在造纸行业也需要用到切片机,适用刀盘式切片机,鼓式切片机,螺旋切片机等。刀盘式切片机由刀盘、机壳、喂料槽以及传动装置等部分组成,工作原理是利用沉重的刀盘起到飞轮的作用,稳定切片。
还有一种是将聚合物带条切成颗粒。这就需要一个特殊的切片机,这种切片机由导条板、喂入辊、加压辊、旋转刀盘组成。工作原理是:利用刀盘由无级变速器传动,喂入辊由刀盘通过一组变换齿轮传动,而刀盘按切粒尺寸装有多把刀片。可以自行调换变换齿轮可改变切断长度和无级变速器可改变带条的惟入速度。向左转|向右转
谢谢!
前段时间用了一下振动切片机,感觉要想准确的切出海马切片不太容易。然后搜索了一下,发现国外用McIlwaintissuechopper这种切片机的人也挺多的,而国内很多都是用的振动切片机。先上两张图
McIlwaintissuechopper
这种切片机的特点是从上向下竖直切下来,每切一次,下面的盘子移动一点点
振动切片机
这种切片机是水平切,将脑组织用502粘在那个小台子上切
两种切片机,我个人的理解是,上面那种可能要更好一点,对于振动切片机,组织块用502粘在小台子上,如果组织块比较大,下面一点点组织浪费也不要紧。但是如果我想切海马,先把海马剥离出来,然后再粘贴在振动切片机上,这样就没办法切了,海马体是个细长的结构,粘上去基本上就全浪费了(我看到很多国内文献都是用的振动切片机,先分离出海马体,然后再切,不知道他们是怎么解决这个问题的,在我看来,1到2周的乳大鼠,分离出的海马体是非常小的,再用胶水粘在小台子上,那都没得切了,不要告诉我说少涂掉胶水,这也解决不了根本问题)但是如果我平放在上面的组织切片机上,从上向下切,这样就没事,可以切出很多切片,完全不会浪费,但还是有个问题,上面那种切片机,虽然每切一次,下面的盘子移动一点距离,但脑组织毕竟是非常柔软的,会不会因为太柔软而不能切的均匀?放在冰水混合物中冻硬一点能不能解决这个问题?
各位大神,因要做切片培养,所以小弟我要用振动切片机切新鲜脑组织,切片厚度为350微米,培养一段时间后,再做免疫荧光染色,但是因为切片比较厚,所以比较难染进去,在论坛里找了很多,全都是5~40μm厚度冰冻切片和石蜡切片的染色技术
有没有谁做过这方面的实验?能否分享一下经验?非常感谢
排除方法:
1.检查电源保证三相正常;和相序正常;
2.测量电压,把电压调整正常;
3.修理、更换接触器、排除线路故障。
4.更换按钮开关
故障2:液压系统压力过低或油缸不动作
排除方法:
1.把电动机转向纠正;
2.修理或更换油泵;
3.把系统压力调整正确;
4.检查吸油管路,更换密封件,排除渗漏;
5.加油至油标中位;
6.清洗或更换吸油或回油滤芯;
7.把油缸接头处的软管拧松,来回运动排气;
8.按要求更换液压油。
故障3:喂料减速机无动作或动作不稳定
排除方法:
1.检查电源保证三相正常;和相序正常;
2.测量电压,把电压调整正常;
3.修理、更换接触器、排除线路故障。
暂无品牌问答