Description
Product Highlights
- Fast – simple protocol for isolation of high quality total RNA, genomic DNA and proteins in as little as 60 minutes
- Efficient – RNA, DNA and protein can be recovered from samples precious or biopsy samples through sequential isolation
- Convenient – an easy-to-use green dye enables easy determination of the phases for improved pipetting accuracy
- Flexible – reagent volume can be scaled for effective extraction of small to large input amounts of cells and tissue
- Consistent – protocol eliminates lysate splitting, thereby improving repeatability of results and increasing yield
Product Description
TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials.
By combining thiocyanate compounds with phenol, TRIsure facilitates disruption of cells during homogenization and effectively inhibits DNase and RNase activity. After homogenizing the sample with TRIsure, chloroform is added and the homogenate is allowed to separate into three phases: a clear aqueous phase (upper) containing the RNA, a green colored organic phase (lower) and an interphase, containing the DNA and proteins. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation, DNA is precipitated from the organic layer with ethanol and the proteins are precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for use in downstream applications.
TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population.
The simplicity of the TRIsure method allows simultaneous processing of a large number of samples and can be used in a wide variety of downstream applications including expression profiling, epigenetics, genotyping, transgenic analysis and cell line characterization.
Applications
- qPCR and RT-qPCR
- End-point PCR and RT-PCR
- Northern/Southern/Western, dot and slot blotting
- Genotyping
- Gene expression analysis
- Transgenic analysis
- Epigenetics
- Cell line characterization
- 2D gel electrophoresis
- Mass spectroscopy
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菜鸟求救,大虾帮忙看看,感激不尽。
总体上来说,在纯水仪器上,进口品牌中密理博、pall、威立雅都算是国内科研单位采购比较多的,当然,售价也较高,一般为国产品牌的3-5倍,除非硬性规定,或者需要用进口高端设备来提升实验室形象,否则,不建议花冤枉钱,因为目前国内很多纯水品牌在技术上都已经追赶甚至超越了进口品牌。
此外,很多人不了解的是,许多欧美品牌的纯水机,虽然技术成熟,但是都是基于国外的水质而研发,而我国自来水水质事实上比欧美国家的低(欧美国家的自来水可直接饮用),因为存在进口品牌的水机在我国水土不服的现象,甚至其前端处理仍需搭配国产设备,只是很多客户并不知晓,尤其是中央供水系统更是如此。这一点需要注意。
国产纯水品牌的话,鱼龙混杂,这里仅仅介绍几个知名度较高,市场做得比较大的,并简单分析对比:
1、锐思捷:在技术上比较成熟,水机的品质和进口品牌做过很多对比和验证,基本上也足以媲美进口品牌了,产品线包含小型机(10-60L)和大型中央供水系统(200L-5T),价格在中高位置;
2、和泰:主做小型机,中央供水系统较少,价格由近万至7万左右,小型机产品线丰富,在市场营销方面下的力度非常大;
3、力康:也算是中高端国产纯水仪器,同时也做其他实验室设备;
此外还有优普、易普易达等区域性品牌。
好?急盼提供建议,多谢多谢!
纯水机,是利用反渗透膜产出纯净水的净水机,属于直饮机。
二者区别:直饮机是一个统称,包含纯水机、超滤直饮机等;纯水机就是生产纯净水的机器,出水可以直接饮用。
RO逆渗透是一种通过目前国际流行的反渗透等办法,对原水进行过滤处理(物理法)后不添加任何化合物而生产出可供人类直接饮用的纯净水机器(也称为终端净水设备)。
采用水质符合中国卫生部《生活饮用水水质卫生规范》(2001)规定的市政自来水为原水,通过2个活性炭滤芯(1个颗粒活性炭、1个烧结活性炭)1个PPF溶喷滤芯对原水进行预过滤,再对预过滤水施加压力令其通过孔径大小为万分之一微米的RO(反渗透,英文Reverse Osmosis)膜,最后通过材质为果壳(椰壳)的载银活性碳(又名小T33)调节水的酸碱度(使制出的纯净水口感变得甘甜醇美)而生产出纯净水。
纯水机的选取首先要看您是做什么用途?不同型号和配置的水机,在各个指标的纯化程度是有倾斜的,这也决定了各型号的水机采取什么核心组件,进而决定价格。例如,如果做的实验室细胞培养类型的,那么就要求有机物、无极离子都要净,价格相对较高;如果是无机分析实验,那么对水中的无机离子去除要求较高,有机物物质相对不那么严格,价格会略低。
同时还取决于设备的产量等因素。
进口的密理博、pall,价格都在5万以上,甚至十多万;国产的比较杂,近万到数万元不等。
国产中锐思捷技术比较好,价格在3-5万之间,其他的如和泰或者乐枫。
暂无品牌问答