Description
Thisrecombinantmulti-epitopechimericHCVantigencontainsthreepeptidesegments.ThefirstsegmentisHCVCorefragment(8-56);itsgenewasPCRmagnifiedfromaclonedplasmidcontainingthefulllengthofHCVgene(type1a).ThesecondsegmentisanimmunodominantregionfromHCVNS4(1916-1947)fragment;itsgenewaschemicallysynthesizedaccordingtotheconsensussequence.ThethirdsegmentistheHCVNS3fragment(1192-1457);itsgenewasextractedfromaclonedplasmidcontainingthefulllengthofHCVgene(type1a).Aftertheywereclonedintoanexpressionvector,pBVIL1,thegeneswereconnectedtogetheronebyoneandexpressedinEscherichiacoliinachimera.TheE.coli-derivedrecombinantproteinwasthenpurifiedbyaseriesofstepstothepurityabove95%asidentifiedbySDS-PAGE.Thefinalproductwaslyophilizedina0.01MTrisbufferpH8.0containing0.02%SDS.(Attention:pleasedissolvejustindistilledwater,mustavoidthepotassiumion)