Description Chromatographicallypurifiedmousealbumin
Productcode MAlb
Biologicalorigin Mouseserum
Physicalform Lyophilized,stablewhitepowder.
Identity Albuminisastablesmallpolypeptidewithastrongantigenicity.Ithas
a molecular weight of 69,000 and an isoelectric point of 5.7,Theproteinisisolated fromserumbysequentialprecipitationandfurtherpurified by ion exchange chromatography and affinitychromatography. The identity is confirmed and tested by immuno-electrophoresis and radial immunodiffusion using antisera to totalserumproteins,immunoglobulins andalbumin.
Purity Notlessthan98%asdeterminedbyagar-gelelectrophoresis.Tested
inaconcentrationof50mg/mlatthelevelofsensitivityoftheappliedimmunoprecipitating techniques, this product contains traces of twoalphalines.
Preservative Nopreservativeadded.
Intendeduse Purified mouse albumin may be used as a reference antigen,
calibrator, coating protein and blocking agent in a variety ofimmunoassays including immunodiffusion, immunoelectrophoresis,ELISA, Western blotting, dot-immunobinding assays (DIBA),haemagglutination,andcell-bindingassays.
Handling This product is shipped lyophilized at ambient temperature. After
arrival store at +4°C. Prolonged storage may be at -20°C. Afteropening thevial, the lyophilizedcontent is reconstitutedbyadding1mlofsteriledistilledwater,mixedgentlyby inversionuntilcompletedissolution isobtained.Allow tostandatambient temperature for5-10 minutes to reach equilibrium. Reconstituted solution may bestored frozen. Working dilutions may be prepared by adding therequiredamountofphosphatebufferedsaline (PBS),shouldnotberefrozenandpreferablyusedwithin24hours.Repeatedthawingandfreezing should be avoided. If a slight precipitation occurs uponstorage, thisshouldbe removedbycentrifugationandwillnotaffecttheperformanceoftheproduct.
Packing Vialwith10mgmousealbuminlyophilizedfrom1mlPBS(pH7.2).
Storage/shelflife
Lyophilizedat+4°C atleast10years
reconstitutedatorbelow-20°C 3-5years
reconstitutedat+4°C 7days
Caution This product should be handled only by qualified persons and
appropriateprecautionsshouldbetakeninitshandlinganddisposal,and of all associated materials. For in vitro laboratory researchpurposesonly.
