Prothrombin is a vitamin K-dependent plasma protein which is synthesized in the liver (1). Prior to secretion into plasma, prothrombin undergoes post-translational modification by a vitamin K-dependent carboxylase which converts ten specific glutamic acid residues to γ-carboxyglutamic acid (gla). The ten gla residues are located within the first 40 amino acids of the mature protein and contribute to the ability of prothrombin to bind to negatively charged phospholipid membranes. Prothrombin contains two regions of internal homology which are referred to as "kringle" structures. These regions of conspicuous secondary structure are located between residues 40 and 270 of the mature plasma protein and replace the growth factor domains found in several other plasma serine proteases. Thus far, no function has been ascribed to these regions, but there is suspicion that they may play a role in one of several binary protein interactions involving prothrombin. The mature single chain protein circulates in plasma as a zymogen and, during coagulation, is proteolytically activated to the potent serine protease α-thrombin. This proteolysis is catalyzed by the prothrombinase enzyme complex. During activation, prothrombin is cleaved at Arg271-Thr272 (human) / Arg273-Thr274 (bovine) and at Arg320-Ser321 (human) / Arg323-Ser324 (bovine) to a "pro" fragment (fragment 1.2) and thrombin, the latter of which is composed of two chains covalently linked by a disulfide bond. In the case of human prothrombin/thrombin, there is an additional thrombin feed-back cleavage at Arg284-Thr285 resulting in an additional 13 amino acids being removed from the mature thrombin “A” chain.
Human prothrombin is prepared from fresh frozen human plasma as described by Bajaj and coworkers (2). Bovine prothrombin is prepared from fresh bovine plasma using a modification of the procedure described by Owen and coworkers (3). Purified prothrombin is supplied in 50% (vol/vol) glycerol/H2O and should be stored at -20oC. Purity is determined by SDS-PAGE analysis, and activity is measured by clotting and/or chromogenic substrate assay, following conversion of prothrombin to thrombin.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
所以说,感染同一种病毒,每个人识别的表位可能不一样。
①乙型肝炎表面抗原—抗体系统(HBsAg/抗—HBs);
②乙型肝炎核心抗原—抗体系统(HBcAg/抗—HBc):
③乙型肝炎e抗原—抗体系统(HBeAg);
④乙型肝炎Dane颗粒抗原—抗体系统
⑤乙型肝炎δ抗原—抗体系统(δ/抗—δ)。
临床意义 1.HBsAg:血清中检测到HBsAg ,表示体内感染了HBV,因而是一种特异性标志。HBsAg阳性见于:①急性乙型肝炎的潜伏期或急性期(大多短期阳性);②HBV致的慢性肝病、迁延性和慢性活动性肝炎、肝炎后肝硬化或原发性肝癌等。③无症状携带者
2.抗HBs:表示曾感染过HBV,不论临床上有无肝炎症状表现,均已得到恢复,并且对HBV有一定的免疫力。
3.HBcAg与抗HBc:由于 HBcAg主要存在于肝细胞核内,并仅存在于Dane颗粒中。因此,对病人血清不能检测HBcAg,而测抗HBc。血清内抗HBc阳性反映:①新近有过HBV感染;②体内有HBV增殖;③有助于诊断急性或慢性乙型肝炎,特别是少数病例就诊时已处于急性恢复期早期,HBsAg已从血中消失,此时血中仅有抗HBc存在,因此,对恢复期患者可作病因追索。
4.HBcAg和抗HBe:HBcAg的存在常表示病人血液有感染性。 HBcAg阳性揭示病人肝脏可能有慢性损害,对预后判断有一定帮助。抗HBe阳性对病人可能有一定的保护力。展开
1.在手机上下载360手机卫士。
2.下载完成手机卫士之后,在手机中安装360手机卫士。在手机中打开360手机卫士并授予手机卫士 Root 权限,如图所示。
3.在手机卫士主界面打开“应用工具”选项,进入后找到“一键 Root 工具”并打开。如果手机已经获取 Root 权限,手机卫士会提示已经手机获取 Root 权限,可以解除 Root 了。
4.点击“解除 Root”按钮之后,360 Root 工具就会给出提示,“注意!解除 Root 之后将永久失去 Root 权限,并且可能无法重新获取!因此若非送修等特殊需要,不建议解除......”确认后点击“解除 Root”按钮即可解除 Root 权限。
方法二:恢复手机的出厂设置。
1.在待机页面下,点击【应用程序】。
2.点击【设定】。
3.向上滑动手机屏幕,点击【重置】。
4.点击【恢复出厂设定】。
5.点击【重置设备】。
6.点击【全部删除】。
7.完成操作后,待手机自动重启后,手机就成功恢复出厂设定了。
核心抗原由183个或185个氨基酸组成,高度磷酸化,是乙肝病毒核心颗粒的唯一结构蛋白。正由于它存在于Dane颗粒核心结构表面,被表面抗原覆盖,故不易在血循环中检出。核心抗原具有强免疫原性,可诱导很强的体液免疫和细胞免疫,刺激机体产生抗-HBc。
e抗原为可溶性蛋白质,传染性强,游离存在于血液中,虽然很早就被发现,在病理上认为是HBV复制以具有强感染性的一个指标,但其功能尚不清楚。抗-HBe的出现,是预后良好的征象。向左转|向右转
主要是中度以上细胞免疫缺陷包括:CD4+T淋巴细胞耗竭,外周血淋巴细胞显著减少,CD4<200/μl,CD4/CD8<1.0,(正常人为1.25~2.1),迟发型变态反应皮试阴性,有丝分裂原刺激反应低下。NK细胞活性下降。
2.各种致病性感染的病原体检查
如用PCR方法检测相关病原体,恶性肿瘤的组织病理学检查。
3.HIV抗体检测
采用酶联免疫吸附法、明胶颗粒凝集试验、免疫荧光检测法、免疫印迹检测法、放射免疫沉淀法等,其中前三项常用于筛选试验,后二者用于确证试验。
4.PCR技术检测HIV病毒。
患者,双手扶墙,脚尖着地;医生做好消毒,戴上手套,利用放血针(可以利用测血糖的放血针),在委中穴点刺放血,利用拔罐器把瘀血抽出来,直到见到新鲜血液为止。委中穴是解毒大穴。
中医强调治症不治病,虽然身体还有病毒,但是,只要症状能够解除,不影响正常生活和工作,就可以了。
需要强调:医生,一定要做好消毒、保护,防止病毒感染。 另外,可以把艾滋病作为一种疫病,尝试五苓散;五苓散是用来治疗疫病的。
暂无品牌问答