PP2A A Subunit
Source: Recombinant Human
Purity: > 90% by SDS-PAGE, apparent Mr ~ 65-kDa
Supplied: 1 μg in 50 μl of 50 mM Tis-HCl pH 7.0 buffer containing 0.1 mM phenylmethanesulfonyl fluoride, 14 mM β-mercaptoethanol, 1 mM EDTA and 50% glycerol. Maintain in aliquots at -70°C. Avoid repeated thawing.
Synonyms: Regulatory 65 kDa A Subunit of Protein Phosphatase 2A; PR65
Background: The A PR65 subunit of PP2A serves as a scaffolding molecule to coordinate the assembly of the catalytic (PP2AC) and variable B subunits. This generates functionally diverse heterotrimers of the phosphatase. The A subunit contains 15 tandemly-repeated HEAT sequences which may participate in its function as a scaffolding molecule. The A subunit also serves to regulate the substrate selectivity of PP2AC and allosterically modulates its polycation response. Association of recombinant A subunit with PP2AC is relatively tight (apparent KD ~ 85 pM) and leads to marked suppression of phosphatase activity with several phosphoproteins substrates. By contrast, suppression of PP2A activity is markedly less with several less-structured or smaller substrates such as phosphopeptides. The affinity of the A subunit for PP2AC is diminished by almost 100-fold following introduction of a glutamate at position 416. This indicates that the basic motif in this region of the protein may be important for its interaction with PP2AC. Some evidence indicates a role for the A subunit in disease. For example, at least four low frequency mutations in genes encoding the A subunit with altered affinity for PP2AC have been identified in several different human cancers. Additionally, overexpression of the A subunit in rat embryo fibroblasts leads to multinucleated cells.
References: Eur J Biochem 267: 5209 Eur J Biochem 248: 200
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1、存在于细胞外环境时，不显复制活性，但保持感染活性，是病毒体或病毒颗粒形式。
2、抗原，是指能够刺激机体产生（特异性）免疫应答，并能与免疫应答产物抗体和致敏淋巴细胞在体外结合，发生免疫效应（特异性反应）的物质
流感病毒的基因组包含________基因,其中__________蛋白可作为__________的结构基础.
我不知道第一空如何填,第二空是否应该填"HA蛋白"?第3空是否应该填"突变"?
多谢各位啊!
新年快乐!
万事如意!
核心抗原由183个或185个氨基酸组成，高度磷酸化，是乙肝病毒核心颗粒的唯一结构蛋白。正由于它存在于Dane颗粒核心结构表面，被表面抗原覆盖，故不易在血循环中检出。核心抗原具有强免疫原性，可诱导很强的体液免疫和细胞免疫，刺激机体产生抗-HBc。
e抗原为可溶性蛋白质，传染性强，游离存在于血液中，虽然很早就被发现，在病理上认为是HBV复制以具有强感染性的一个指标，但其功能尚不清楚。抗-HBe的出现，是预后良好的征象。向左转|向右转
所以说，感染同一种病毒，每个人识别的表位可能不一样。
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