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UnitDefinition | OneWeissunitofT4DNALigaseconverts1nmoleofp32frompyrophosphateintoNorit-adsorbablematerialin20minutesat37℃in33mMTris-acetate(pH7.8),66mMpotassiumacetate,10mMmagnesiumacetate,0.5mMDTT,and1mMATP.OneWeissunitequalsapproximately200cohesive-endligationunits. |
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QualityControl | T4DNALigaseisfunctionallytestedincloningassaysandisfreeofdetectablecontaminatingDNAexo-andendo-nucleaseandRNaseactivities. |
Concentration | 1Weissunit/㎕ |
StorageBuffer | 50mMTris-HCl(pH7.5),0.1MNaCl,0.1mMEDTA,0.1%TritonX-100,and1mMDTT,50%Glycerol |
10XReactionbuffer | composedof400mMTris-HCl,100mMMgCl2,100mMDTT,5mMATP(pH7.8at25°C). |
Application | Cloningofrestrictionfragments |
Joininglinkersandadapterstoblunt-endedDNA |