Product Name | HiLyteᵀᴹFluor 488 hydroxylamine, HCl salt *single isomer*HiLyte Fluor 488 C2 - aminooxyacetamide, HCl salt *single isomer* |
Size | 1 mg |
Catalog # | AS-64348 |
US$ | $342 |
HiLyte Fluor 488 hydroxylamine is a carbonyl-reactive labeling dye, which reacts more readily with aldehydes at physiological pH than other primary amine-containing reagents (such as hydrazides and amines). The reaction of HiLyte Fluor 488 hydroxylamine with aldehydes yields oxime ethers, which are stable at physiological conditions. Therefore, HiLyte Fluor 488 hydroxylamine can be used to directly detect aldehydes in proteins and DNA.Extinction Coefficient (M-1cm-1): 70,000Fluorescence quantum yield: 0.91Fluorescence Life Time (ns): 4.1 | |
Detailed Information | Material Safety Data Sheets (MSDS) |
Storage | -20°C desiccated and protected from light |
References | D. Boturyn, et al, Tetrahedron, 53, 5485 (1997); J. G. Hecker, et al, ChemmedChem, 3, 1356 (2008); Y. Zeng, et al, Nature methods, 6, 207 (2009). |
Molecular Weight | 525.94 |
Spectral Properties | Abs/Em = 498/525 nm |
Solvent System | DMF or DMSO |
Product Citations | Fabrini R. et al. Biochemistry. 2009 Oct 9 |
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而且比较容易买到,同时提取的DNA便于纯化
buffer P1:除去RNA
buffer P2:裂解细胞
buffer P3:沉淀DNA
buffer WA、buffer WB:都是洗涤液(这两个之间有什么区别我也不清楚)
TE:溶解DNA.
哪个正规生物公司DNA提取试剂好用,纯,效果好
大家都用过哪些DNA提取试剂盒,能不能推荐几种
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