- Directreplacementsforstandardcloningstrains(e.g.,DH5α™,DH10B™,JM109,TOP10,XL1-Blue,etc.)
- Optimizedgeneticsforhighyields: phageT1resistant,endonucleaseandrecombinationminus,blue/whitescreening-capable.
- Availableinarangeofhightransformationefficiencies(5×109to4×1010cfu/µg).
- Convenientpackagingoptions.
Lookingforchemicallycompetentversion?
FreeSampleAvailable
Lucigen’sE.clonicompetentcellssharethemostusefulgeneticelementsofstandardcloningstrainslikeDH5α™,DH10B™,JM109,TOP10,etc.anddirectlyreplacethemincloningprotocols.However,E.clonielectrocompetentcellsincorporateauniquemanufacturingtechnologythatincreasestransformationefficiency,recombinantyieldsandreliABIlity(Figures1and2),whiledecreasingcosts. Thesecellsprovidesolutionsforawiderangeofapplicationsateconomicalprices.
Choiceofstrain:
- E.cloni10GCompetentCells.Libraryconstruction,cloning,subcloning,andplasmidisolationwithorwithoutblue/whitescreening.
- E.cloni10GF′CompetentCells.ContaintheF'plasmidforinfectionwithM13toproducessDNA.
Choiceofefficiency:
- E.cloni10GSUPREMEElectrocompetentCells>4×1010cfu/µgpUCDNA
SUPREMECellshavethehighesttransformationefficiencyavailablefromanysupplier.ChooseSUPREMECellsforthemostdemandingcloningsituations,suchasconstructionoflarge,highcomplexitylibrariesorcloningdifficulttargets,whichrequirethegreatestnumberoftransformantspossIBLe. - E.cloni10G&10GF´ELITEElectrocompetentCells>2×1010cfu/µgpUCDNA
ELITECellshavetwicethetransformationefficiencycomparedto“ultrahighefficiency”cellsfromothersuppliers.ELITECellsprovidelargenumbersoftransformantsfromhard-to-clonefragmentsorlimitedDNAatalowerprice. - E.cloni10GCLASSICElectrocompetentCells>5×109cfu/µgpUCDNA
CLASSICCellsarehighefficiencycellswithasubstantiallylowercostperreaction.Thesecellsarethemosteconomicalchoiceforstandardcloningandlibraryconstruction.10GCLASSICCellsareavailableinlargerpackagesizesforconvenientuseinhighervolumecloningapplications.
CustomCompetentCells
BulkLucigencellsoryourstrainofE.coli,madetothehighestpossibletransfectionefficiencywithcustomdispensing!SeeCustomCompetentCellspagefordetailedinformation.
Plasmidcloning.E.cloni10GSUPREMECellsproviderecombinantyieldshigherthanthehighestefficiencycellsofferedbyaleADIngsupplier(Figures1and2)
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Figure1.E.cloni10GSUPREMEElectrocompetentCellsconsistentlyoutperformed“ultrahighefficiency”cellsfromaleadingsupplier.Bothstrainsweretransformedwith10pgofpUC19(n=16). |
| Figure2.TransformationefficiencycomparisonofE.cloni10GSUPREMECells(Lucigen)andMegaDH10Bcells(Invitrogen).Competentcellsweretransformedaccordingtothemanufacturer’sinstructionswiththesamepUC19DNAcontrol(providedwiththeInvitrogencells) |
Table1.E.cloniCompetentCellsforCloningandLibraryConstruction
E.cloniCellLines | TransformationEfficiency | CloningMethylatedDNA | BAC,CosmidCloning | Blue/WhiteScreening |
10GSUPREMEElectrocompetent | >4×1010 | YES | YES | YES |
10GELITEElectrocompetent | >2×1010 | YES | NO | YES |
10GCLASSICElectrocompetent | >5×109 | YES | NO | YES |
10GF´ELITEElectrocompetent | >2×1010 | YES | NO | YES |
Efficiencies
- E.cloni 10GSUPREMEElectrocompetentCells > 4×1010 cfu/µgpUCDNA
- E.cloni 10G&10GF´ELITEElectrocompetentCells > 2 × 1010 cfu/µgpUCDNA
- E.cloni 10GCLASSICElectrocompetentCells > 5 × 109 cfu/µgpUCDNA
ORDERINFORMATION
CompetentCellsincludeControlDNAandRecoveryMedium,andarepackagedasSOLOs(1transformationpertube),DUOs(2transformationspertube),orSixPacks(6transformationspertube).RecoveryMediumisalsoavailableseparately.ThespecifiedtransformationefficienciesarewithpUCDNA,unlessindicatedotherwise.
PLEASENOTE:Bulkquantities(10timeslargerthanthelargestretailpackagesizebelow)and/orcustompackagingareavailableatveryattractivepricesforallLucigenCompetentCells.PleasecontactLucigen.
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在细菌及古细菌中，CRISPR系统共分成3 类，其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白（Cas蛋白）共同发挥作用，而Ⅱ类系统 。
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