商品信息
联系客服
郑重提醒：
无质量问题不接受退换货，下单前请仔细核对信息。
下单后请及时联系客服核对商品价格，订单生效后再付款。
Specifications
Print Version
| Description | These 0610010F05Rik protein vectors can be used for high level expression of the 0610010F05Rik protein. Protein vectors are available for expressing from bacterial or mammalian cells with a variety of tags for easy purification. |
|---|---|
| SKU | 1001002 |
| Gene Name | 0610010F05Rik |
| Unit quantity | 500 ng |
| Aliases | Kiaa1841; mKIAA1841; RP23-188K3.6 |
| Accession Number | NM_027860 |
| Vector Map | pPB-C-His, pPB-His-GST, pPB-His-MBP, pPB-N-His, pPM-C-HA, pPM-C-His, pPM-N-D-C-HA, pPM-N-D-C-His |
| Appearance | Clear liquid |
| Insert Size | NM_027860:2157NM_027860:4140 |
| Storage Condition | 1 year when stored at -20°C or lower in a non-frost free freezer. |
| Storage Buffer | 10mM Tris-HCI, 1mM EDTA, pH8.0 |
| Vector Size | pPB-C-His:5246bppPB-N-His:5307bppPM-C-HA:4765bppPM-C-His:4752bppPB-His-MBP:6421bppPB-His-GST:6000bppPM-N-D-C-HA:4808bppPM-N-D-C-His:4797bp |
| Species | This gene is available from: Mouse |
| Bacterial Selection | Kanamycin |
| Accession Number | NM_027860 |
| Guarantee | abm guarantees that the correct ORF construct is provided and the mRNA expression is displayed upon successful transduction. If this is not the case, we will provide a one-time replacement. Customers must provide adequate data to show >80% transfection efficiency with a positive control, plus additional qPCR data to evaluate the level of gene expression. The replacement will not be covered by the same guarantee.Please note that due to the large number of variables applicable, any further expression analysis (e.g. protein expression) is not covered by the guarantee, as such analysis is dependent on the end user"s experimental conditions. |
| Disclaimer | 1) Disclaimer for Transcript Variants: The provided accession number refers to the transcript (mRNA) sequence for this product. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. All sales are final.2) Disclaimer for Gene Sequence: The provided accession number refers to the transcript (mRNA) sequence for this product. Please verify that this is the desired transcript sequence by cross-referencing. This is important because a single gene can have multiple different transcripts owing to naturally occurring variations. All sales are final.3) Disclaimer for Intended Use: All of abm"s vectors and viral particles are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its vector(s) in therapeutic/diagnostic application(s).4) Disclaimer for Extra Nucleotides: Cloning may lead to the insertion of extra nucleotides at the 5" or 3" end of the target sequence which, in most cases, is innocuous to the stability/functionality of the construct.5) abm guarantees that at least 1 out of the 3 sgRNA constructs purchased in a set designed to be used with Cas9 Nuclease will result in gene knock-out due to frameshift mutations in over 50% of cells, after successful infection and drug selection. This guarantee applies to sgRNAs designed to target human, mouse or rat genes only. If knock-out is not achieved in extremely rare cases, a one-time replacement of another set of 3 targets with alternative sgRNA sequences will be provided. To qualify for this replacement, customers must examine knock-out efficiency by Surveyor assay. Before sending your inquiry, please make sure you have optimized your experiments as far as possible. This includes (where applicable) increasing and optimizing your MOI, increasing the duration of infection (up to 72 h), and carrying out clone screening before assaying for knock-out. Please also provide data to show that a reporter virus was used to optimize the MOI for your target cell line. Customers must provide adequate data to show >80% infection efficiency with a positive control, plus additional qPCR data to evaluate the level of mRNA expression.For vector transfection, please evaluate the vector transfection efficiency by detecting Cas9 or puromycin expression for the "All-in-One" vectors using qPCR, or neomycin for constructs containing only the sgRNA. In addition, please provide Surveyor Assay or Sanger Sequencing data on at least 20 isolated clones.abm limits its obligation and liability for the success of this technology to providing one replacement of any sgRNA lentivector product only. The replacement set will not be covered by the same guarantee. If these constructs are also considered to be ineffective then the gene is most likely not susceptible to sgRNA knock-out. |
Documents
- Protein Production Guideline using pPB Vectors
- Protein Vector Amplification
- Protein Vector FAQ
FAQs
| What is the difference between Retro-, Lenti-, and Adeno- viruses? | |
Retrovirus: Classic, can integrate into the genome but with low transduction efficiency. They are useful for gene transfer and protein expression in cells that have low transfection efficiency with other transfection reagents.Lentivirus: Can integrate into the genome with relatively high transduction efficiency and they are very useful for cells that have low transfection efficiency with other transfection reagents. No special competent cells required, as they are stable plasmids. Lentiviruses are a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo.Adenovirus: Only work transiently (about 7 days) but have almost 100% transduction efficiency. Adenoviruses can infect a broad range of cell types with the highest efficiency and infection is not dependent on active host cell division. A second key feature is that high virus titers and high-level gene expression can be obtained in most mammalian cells. | |
| What are the correct concentration units for each recombinant viral particle? | |
For lentiviruses and retroviruses, they are measured in CFU/ml (colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. For AAV the titer is measured as genome copies per mL (GC/mL). Adenoviruses are measured as PFU/ml (plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. The concentration for all three types of viruses can also be classified as IU/ml (Infectious Units/ml). Ultimately, the units refers to the viral particles and different units reflect the different assays involved. | |
| How long after transduction can the infection efficiency be observed? | |
You can observe transduction efficiency from 48 hours up to 5 days after infection. | |
References
- Wang, Q., Yu, H., Yu, H., Ma, M., Ma, Y., & Li, R. "miR‑223‑3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in�vitro" Molecular Medicine Reports. : (2018). DOI: 10.3892/mmr.2018.9742.
- Xu, T., He, B. S., Pan, B., Pan, Y. Q., Sun, H. L., Liu, X. X., ... & Wang, S. K. "MiR‐142‐3p functions as a tumor suppressor by targeting RAC1/PAK1 pathway in breast cancer" Journal of Cellular Physiology. : (2019).
- Zhang, B., Roosmalen, I. A. M., Reis, C. R., Setroikromo, R., & Quax, W. J. "Death receptor 5 is activated by fucosylation in colon cancer cells" The FEBS Journal 286(3):555–571 (2019). DOI: 10.1111/febs.14742.
- Zhao, Q., Zhao, S., Li, J., Zhang, H., Qian, C., Wang, H., … Zhao, Y. "TCF7L2 activated HOXA-AS2 decreased the glucocorticoid sensitivity in acute lymphoblastic leukemia through regulating HOXA3/EGFR/Ras/Raf/MEK/ERK pathway" Biomedicine & Pharmacotherapy 109:1640–1649 (2019). DOI: 10.1016/j.biopha.2018.10.046.
蚂蚁淘电商平台
ebiomall.com
ebiomall.com
公司简介
蚂蚁淘（www.ebiomall.cn）是中国大陆目前唯一的生物医疗科研用品B2B跨境交易平台，
该平台由多位经验丰富的生物人和IT人负责运营。蚂蚁淘B2B模式是指客户有采购意向后在蚂蚁
淘搜索全球供应信息，找到合适的产品后在蚂蚁淘下单，然后蚂蚁淘的海外买手进行跨境采购、
运输到中国口岸，最后由蚂蚁淘国内团队报关运输给客户...
蚂蚁淘承诺
正品保证： 全球直采 在线追溯
蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权； 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程， 确保货物的来源； 正规报关，提供13%增值税发票。
及时交付： 限时必达 畅选无忧
蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节； 同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上， 部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。
轻松采购： 在线下单 简单省事
蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价； 报价单与合同可以直接在线生成或打印；就像在京东购物一样， 您的鼠标点击几 次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。
售后申请： 耐心讲解 优质服务
蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时， 您可通过我的订单提交您的“申请售后”， 蚂蚁淘产品顾问会第一时间为您处理， 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。
2021-08-25
导读：近日，广州市妇女儿童医疗中心珠江新城院区眼科副主任朱洁博士科研团队发表了一项突破性科研成果，他们通过对视细胞进行基因编辑，能有效防止视网膜细胞变性，并保留视网膜组织感光功能，有望帮助视网膜色素变性的患者摆脱失明的阴影。 视网膜色素变性是造成失明的主要原因之一，目前临床上还没有有效的治疗手段，患者常出现夜盲、视野缩小，最终视力丧失，严重影响生活质量。 近日，广州市妇女儿童医疗中心珠江新城院区眼科副主任朱洁博士科研团队发... 查看更多
>
2018-06-28
巴傲得生物长期备有Bioworld抗体现货4000多种抗体可以在一周左右送到客户...上海遐锦生物科技有限公司于2019年11月29日举办全国循证医学Meta分析与网状... 查看更多
>
2017-07-30
据国外媒体报道，半个多世纪以来，科学家一直梦想着利用“自私的基因”对所有物种进行基因编辑。这种基因可谓自然界的一大怪胎，它竟能绕开正常的遗传法则、强行把自己遗传给下一代。几年前，伴随着 CRISPR-Cas9 基因编辑技术的问世，使这一带有科幻小说色彩的理念变成了触手可及的现实，名为“基因驱动技术”（gene drive）。但除了媒体的炒作和对滥用该技术的恐惧，科学家如今对基因驱动技术的实际作用提出了怀疑。基因驱动技术是一种分子技术，可 查看更多
>
2017-09-06
微生物与人体细胞之间通过信号分子实现密切交流：如配体与膜结合型 G 蛋白耦联受体（GPCR）的相互作用。
为了了解这些“神秘”的配体，Rockefeller 大学小分子基因编码研究室主任 Sean Brady 领导了 Rockefeller 大学和西奈山 Icahn 医学院的科研人员进行了相关研究，具体内容于 8 月 30 日发表在 Nature 期刊，题为“Commensal Bacteria Make GPCR Ligands That Mimic Human Signalling Molecu 查看更多
>
2021-08-07
上海吉凯基因化学技术有限公司在发布的基因编辑大小鼠服务供应信息，浏览与基因编辑大小鼠服务相关的产品或在搜索更多与基因编辑大小鼠服务相关的内容。 查看更多
>
基因编辑学术研讨会支持媒体: http://www.infobio.cn 中国生物信息网会展中心学术会议栏目为广大仪器企业提供基因编辑学术研讨会预告、会议详细信息。仪器网，致力打造仪器行业互动门户。 查看更多
>
2017-06-07
生物黑客进军基因编辑领域。 查看更多
>
2017-06-29
细胞培养,支原体检测和清除,微生物和植物培养,血清,澳洲血清,胎牛血清,特级胎牛血清,ausbian胎牛血清 查看更多
>
2021-08-26
导读：近日，广州市妇女儿童医疗中心珠江新城院区眼科副主任朱洁博士科研团队发表了一项突破性科研成果，他们通过对视细胞进行基因编辑，能有效防止视网膜细胞变性，并保留视网膜组织感光功能，有望帮助视网膜色素变性的患者摆脱失明的阴影。 视网膜色素变性是造成失明的主要原因之一，目前临床上还没有有效的治疗手段，患者常出现夜盲、视野缩小，最终视力丧失，严重影响生活质量。 近日，广州市妇女儿童医疗中心珠江新城院区眼科副主任朱洁博士科研团队发... 查看更多
>
2018-01-29
直到十年前，科学家们还没有意识到细菌具有复杂的免疫系统，即能够跟上感染细菌的病毒（即噬菌体）进化速度的免疫系统。随着发现一种如今最为知名的被称作CRISPR的细菌免疫机制以后，情况发生了变化。科学家们已意识到CRISPR是一种天然的基因编辑器，而且它已在世界各地数以千计的实验室中引发生物学研究领域变革。如今，研究人员理解到大多数微生物具有复杂的免疫系统，而CRISPR仅是其中的一个组分；但是没有一种很好的方法来鉴定这些系统。在一项大... 查看更多
>
2018-02-22
因为使用方便、构建简单，CRISPR 被认为是遗传研究领域的革命性技术。如今，这个热门基因编辑工具不仅被用于改造哺乳动物细胞，从农业到医疗甚至到环境保护（麻省理工教授张锋： CRISPR 将在气候问题上大放异彩），科学家们都对它表现出极浓的兴趣。而在 CRISPR 的研究上，有两个先锋人物我们不得不提，那就是麻省理工学院教授张锋和加州大学伯克利分校教授 Jennifer Doudna。图丨张锋和 Jennifer DoudnaJenni 查看更多
>
常见问题
蚂蚁淘所售产品均为正品吗？
蚂蚁淘的创始人兼CEO是钟定松先生，具有十年的从业经验，在业界享有良好的口碑；
Ebiomall是跨境直采平台，我们直接从厂家采购，自己的团队负责国际物流和清关，中间没有第三方，蚂蚁淘承诺所售产品仅为正品，假一罚十。
下单后可以修改订单吗？
未确认状态的订单可以修改，打开“订单详情”页面，点击右上角的“修改订单”即可，若已审核确定，则订单无法修改。
商品几天可以发货？
现货产品付款审核后即可发货,大部分期货产品在3周左右即可到货,提供时必达服务的产品订单审核十天内即可发货。
订单如何取消？
如订单处于未确定状态，进入“我的订单"页面，找到要取消的订单，点击“取消订单”按钮。
可以开发票吗？
本网站所售商品都是正规清关，均开具13%正规发票，发票金额含配送费金额，另有说明的除外。
如何联系商家？
蚂蚁淘任何页面都有在线咨询功能，点击“联系客服”、“咨询”或“在线咨询”按钮，均可咨询蚂蚁淘在线客服人员，
或拨打4000-520-616，除此之外客户可在 联系我们页面找到更多的联系方式。
收到的商品少了/发错了怎么办？
同个订单购买多个商品可能会分为一个以上包裹发出，可能不会同时送达，建议查看订单详情是否是部分发货状态；如未收到，可联系在线客服或者致电4000-520-616。
退换货/维修需要多长时间？
一般情况下，退货处理周期为客户收到产品一个月内（以快递公司显示签收时间为准），包装规格、数量、品种不符，外观毁损、短缺或缺陷，请在收到货24小时内申请退换货；特殊商品以合同条款为准。
商品咨询
crispr cas9 蛋白还表达怎么办_问答详情_CRISPR 细胞株_基因编辑...123
灰机52gOd2017-10-01
CRISPR(clustered,regularlyinterspaced,shortpalindromicrepeats)是一种来自细菌降解入侵的病毒DNA或其他外源DNA的免疫机制。
在细菌及古细菌中，CRISPR系统共分成3 类，其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白（Cas蛋白）共同发挥作用，而Ⅱ类系统 。
在细菌及古细菌中，CRISPR系统共分成3 类，其中Ⅰ类和Ⅲ类需要多种CRISPR相关蛋白（Cas蛋白）共同发挥作用，而Ⅱ类系统 。
▍
品牌分类
▍
官网分类
▍
品牌问答
暂无品牌问答
