Highpurityrecombinantendo-α-N-Acetylgalactosaminidase(Enterococcusfaecalis)foruseinresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
EC3.2.1.97
CAZyFamily:GH101
CAS:59793-96-3
endo-alpha-N-acetylgalactosaminidase;glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamineD-galactosyl-N-acetyl-galactosaminohydrolase
Recombinant.FromEnterococcusfaecalis.
In20mMTris.HClpH7.5,50mMNaCl,5mMEDTA.
Suppliedat~1U/mL.
Specificactivity:
~2U/mg(37oC,pH7.5onGal-β-1,3-GalNAc-α-pNP).
StABIlity:>2yearsat-20oC.
Customquantitiesandformulationsavailableuponrequest.
DESCRIPTION
endo-α-N-Acetylgalactosaminidase(Enterococcusfaecalis)
EC3.2.1.97
CAZyFamily:GH101
CAS:59793-96-3
Synonyms:
endo-alpha-N-acetylgalactosaminidase;glycopeptide-D-galactosyl-N-acetyl-alpha-D-galactosamineD-galactosyl-N-acetyl-galactosaminohydrolase
Form:
In20mMTris.HClpH7.5,50mMNaCl,5mMEDTA.
Stability:
>2yearsat-20oC.
Specificactivity:
~2U/mg(37oC,pH7.5onGal-β-1,3-GalNAc-α-pNP).
Unitdefinition:
OneUnitofendo-α-N-acetylgalactosaminidaseactivityisdefinedastheamountofenzymerequiredtoreleaseoneµmoleofp-nitrophenolperminutefromGal-β1,3-GalNAc-α-pNP(0.2mM)inTris.HClbuffer(100mM)pH7.5at37oC.
Specificity:
HydrolysisoftheO-glycosidic-α-linkagebetweenGal-β-1,3-GalNAc(Core1)orGlcNAc-β-1,3GalNAc(Core3)andaserineorthreonineresidueofglycopeptidesandglycoproteins.
Applications:
Applicationsinclinicalchemistry,glycoBIOLOGyandcarbohydrateresearch.
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请教各位老师,
文献中检测细胞的活性有的用“NADPHdehydrogenase(NADPH脱氢酶)”有的用“NADPHdiaphorase(NADPH黄递酶)”
它们是同一种酶吗?它们的功能是什么?检测它们的活性是否能够评估细胞的活性。
不胜感激
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
明白了么,少年?
有谁用碧云天的过氧化氢酶检测试剂盒,在试剂盒的准备工作中,过氧化氢的实际浓度=22.94*A240,我测出来的吸光度是3.3左右,那么乘以22.94就等于76多点,再乘以之前的稀释倍数,大约就是7600mM左右,这样跟说明书中说道的1M相差太多,感觉不对啊,稀释的肯定是没有错的,但是不知道哪里出了错,怀疑说明书就有问题呢,有人做过这个实验吗?能不能准确测出过氧化氢浓度吗?有测过的人帮忙指点一下啊,不知道应该怎么做