CRESYL FAST VIOLET TECHNIQUE FOR NISSL
来自 : 蚂蚁淘
SECTIONS
8µmparaffinwaxsections
SOLUTIONS
STAININGSOLUTION
- CresylFastViolet-1g
- Distilledwater-100cm3
- Aceticacid-0.25cm3
METHOD
- Takesectionstowater
- Stainincresylfastvioletsolutionfor20-30minutes
- Rinseindistilledwater
- Leavein96%alcoholuntilmostofthestainhasbeenremoved
- Clearinxylene.MountsectionsinDPX
RESULTS
- Nisslsubstance-Purple/darkblue
- Neuronesandcellnuclei-Purple/blue
NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.
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