Description:
KAPAStrandedRNA-SeqKitwithRiboErase(HMR)KAPAStrandedRNA-SeqwithRiboErase
Evolvedtofocus.
TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.
- Upto99.98%rRNAdepletion*
- FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
- Robustandreproducibleacrossvariousinputamounts
- Automation-friendlyworkflow
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
DownloadourAdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
IndustryleADIngrRNAdepletion
- SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
- MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest
Unsurpassedsequencingdataquality
- Detectionofmoregenesanduniquetranscripts
- Accurateandclearidentificationofsplicesitesandalternativegenesplicing
- ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts
- 0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">
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Highlyreproduciblesequencingresults
- Highcorrelationevenbetweendifferenttestingconditions
- Lowsample-to-samplevariationformorereliableresults
Improvedcoverageuniformity
- Uniformdistributionofreadsovereachtranscript
- Minimal5’–3’biasacrosstranscripts
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
Applications:
Applications- WholeTranscriptome
- GeneExpression
- TranscriptDiscoveryandAnnotation
- TotalRNA-Seq
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto10monthsat-20ºC.
KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).
Components
Specifications
- SpecDescription
- CompatiblePlatformIlluminaHiSeq®,NextSeq®andMiSeq®
- LibraryTypeRNA
- StartingMaterialHigh-qualityTotalRNA
- InputAmount100ng-1µg
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实验非常不顺,想构建CDNA文库,但是从mRNA开始屡次失败,考虑主要是纯化过程中损失过多。想从总RNA入手,但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶,苦于没有第二连合成试剂盒,不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时,不需要另外的引物,利用降解的RNA作引物即可,我想直接设定PCR两个循环,合成第二链,不知方法可行否?愁啊,等着毕业,时间紧急,恳请帮忙。谢谢。
mRNA 数量不详,根据转录数量,加工数量各不相同,故无法得知。
用醌指纹法描述微生物群落的参数[7]有:(1)醌的类型和不同类型的醌的数目;(2)占优势的醌及其摩尔分数含量;(3)总的泛醌和总的甲基萘醌的摩尔分数之比;(4)醌的多样性和均匀性;(5)醌的总量等。对两个不同的群落,由上述分析所得数据可以计算出另一个参数____非相似性指数(D),用于定量比较两个群落结构的差异。
醌指纹法具有简单快速的特点,近几年来广泛用于各种环境微生物样品(如土壤,活性污泥和其它水生环境群落)的分析。
植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
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