Bovine Brain Stem Total RNA is extracted from freshly harvested tissues of single healthy normal animal donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase-1to remove residual DNA, precisely quantified, and stored at -80oC.
Quality Control: The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by spectrophotometer (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR.
Applications: RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction.
Packing/shipping: Total RNA sample is routinely provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice. Sample can also be provided in 1mM sodium citrate, 0.1 mM EDTA, or any other RNA storage buffer.
MSDS and Certificate of Analysis in PDF files: Contact Zyagen Technical Support at zinfo@zyagen.com
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试验中的“空白试验”,是指在不加供试品或以等量溶剂替代供试液的情况下,按同法操作所得的结果。其作用是排除实验的环境（空气、湿度...）、实验所用的药品（指示剂等等）实验操作（误差、滴定终点判断之类的）对实验结果的影响。
试验中的“空白试验”,系指在不加供试品或以等量溶剂替代供试液的情况下,按同法操作所得的结果;含量测定中的“并将滴定的结果用空白试验校正”,系指按供试品所耗滴定液的量(ml)与空白试验中所耗滴定液量(ml)之差进行计算。
标准品，即标准物品，是中药标准对照品研究中心代理的作为一种衡量标准；用做药物方面，则为含量测定中的标准含量。标准品包括化学计量标准品、冶金标准品和药检标准品。
我做实验的药物很难买到标准品，但现在有粗品，自己在提纯，想问下各位大牛，在没有标准品的情况下，怎么测提纯后该物质的纯度。谢谢
各们前辈，初涉诊断行业，开发PCR诊断试剂，求问微生物标准品或者质控血清（比如支原体、衣原体、金葡菌等）应该到哪里购买啊？中检所和卫生部临检中心好像都没有。谢谢！
Reference material, accompanied by a certificate, one or more of whose property values are certified by a procedure, which established its traceability to an accurate realization of the unit in which the property values are expressed, and for which each certified value is accompanied by an uncertainty at a stated level of confidence. (Brammer StandardBASARMIetc). (ISO Guide 30-1992)
标准品CRM：标准物质/标准品附带析证书属性值某项或若干项都按照某程序进行验证验证程序建立些属性值所表述体系精确体现追溯性每经验证数值都伴随着某特定置信水平确定度（BS,NISTBASARMI等）（ISO Guide 30-1992）
Reference Materials（RM）
Materials or substance one or more of whose property values are sufficiently homogeneous and well established to be used for the calibration of an apparatus, the assessment of a measurement method, or for assigning values to materials. （ISO Guide 30-1992）
照品RM：种原料或物质其某种或几种属性值表现充均性并且用于设备校准析评估或者物质定植（ISO Guide 30-1992）
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