Mighty Cloning Reagent Set (Blunt End) is designed to allow PCR products to be quickly cloned into blunt-end vectors with a simple method. The blunting and 5"-phosphorylation reactions are done simultaneously and in parallel, so both blunt- and -dA ended products can be prepared in a single reaction. This kit eliminates the need for pretreatment (such as enzyme inactivation, removal of unused dNTPs and primers, etc.) of PCR products.
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蛋白的类型为PDGF。其有一种二聚体为PDGF-BB。
请教第一点:我看了Elisa的说明书,现在检测PDGF有两种试剂盒,一种是检测PDGF的,要求的试剂样本为组织匀浆;一种是检测PDGF-BB的,要求的待测样本为血液或血浆。
我现在的实验目的是想更精确,检验组织中的PDGF-BB,但是手中的是人体韧带组织样本,非PDGF-BB试剂盒所要求的组织样本为血浆或血清,我不知道是否可以用PDGF-BB的ELISA试剂盒的来做,所以特地请教:)
请教第二点:市面上有RB公司的96T,2500元;另外有Uscnlife的Elisa试剂盒,96T的3680元,都说是原装进口的,我该如何选择呢?为何差价这么多?谢谢指点!
上海好多的供应商的,具体还是看你选择了,其实试剂盒产品都差不多,什么远慕生物,古朵生物...好多的额
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