Cystatin C Rapid Human ELISA Kit
For the quantitative determination of human cystatin C concentrations in serum, plasma and urine samplesHuman cystatin C (or cystatin 3), which is composed of 120 amino acid residues, belongs to the cystatins superfamilly that inactivates lysosomal cysteine proteinases. As a strongly cationic and low-molecular weight (13.4 kDa) protein, it is almost freely filtered across the glomerular membrane, and is mainly used as a biomarker of kidney function. A growing body of evidence suggests that cystatin C is a more reliable biomarker of glomerular filtration rate than creatinine [1-3]. In addition to kidney disease, altered serum levels of cystatin C are associated with several types of cardiovascular disease, including myocardial infarction, stroke, heart failure, peripheral arterial disease and metabolic syndrome [4-7]. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer"s disease [8, 9]. Furthermore, Cystatin C has also been investigated as a prognostic marker in several forms of cancer [11, 12].REFERENCES [1] Stevens LA, Coresh J, Schmid CH, et al. (2008) Am J Kidney Dis. 51:395–406. [2] Dharnidharka VR, Kwon C, Stevens G. (2002) Am. J. Kidney Dis. 40 (2): 221–6. [3] Hermida J, Tutor JC. (2006) Ther Drug Monit. 28 (3): 326–31. [4] Zethelius B, Berglund L, Sundström J, et al. (2008) N. Engl. J. Med. 358 (20): 2107–16. [5] Ix JH, Shlipak MG, Chertow GM, Whooley MA. (2007) Circulation 115 (2): 173–9. [6] Deo R, Fyr CL, Fried LF, et al. (January 2008) Am. Heart J. 155 (1): 62–8. [7] Servais A, Giral P, Bernard M, et al. (2008) Am. J. Med. 121 (5): 426–32. [8]Mi W, Pawlik M, Sastre M, et al. (2007) Nat. Genet. 39 (12): 1440–2. [9] Kaeser SA, Herzig MC, Coomaraswamy J, et al. (2007) Nat. Genet. 39 (12): 1437–9. [10] Zurdel J, Finckh U, Menzer G, et al. (2002) Br J Ophthalmol 86 (2): 214–9. [11] Strojan P, Oblak I, Svetic B, et al. (2004) Br. J. Cancer 90 (10): 1961–8. [12] Kos J, Krasovec M, Cimerman N, et al. (2000) Clin. Cancer Res. 6 (2): 505–11.
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1)做免疫组化的片子最好不要同时做HE染色,因为那样会掩盖阳性着色的结果;而且用苏木素复染细胞核也是淡染,若要在免疫组化的片子上观察形态学的改变,除非有非常典型的表现,一般是有很大的难度的。
2)你是培养的细胞做免疫组化吗?如果是的话,那就每个样本只有一张片子吧?唯一的办法是进行免疫双染,同时做CD34和AFP(甲胎蛋白)。我觉得后者阳性便可以或多或少的获得向肝细胞分化的证据。
3)用HE染色来识别造血干细胞向肝细胞分化,也就是说要看到肝细胞的典型形态学改变后才可以下结论。问题是:早期的肝细胞仅凭形细胞态学观察并不能很容易的被识别。病理大夫看组织切片,重要的是看其组织学结构,对肝细胞也是这样。如果单拿出来一个细胞,还是培养的细胞,染色后真的不容易区分。所以我觉得你设计的用HE染色来确定早期细胞向哪个方向分化不是很有说服力。
4)如果是切片,也就是说每个样本可以有多张切片,那可以在连续切片上分别染CD34和AFP,拍照时选择相同视野,也有一定说服力。但是还是不如双染法更可靠。
5)用双染最大的问题在于:这两者都表达在胞浆中,染色容易相互覆盖。所以在双染的方法选择上你还要再考虑一下,可不可以用免疫荧光双染?这样也很有说服力的
有没有朋友使用碧云天的细胞衰老β-半乳糖苷酶染色试剂盒做过细胞衰老,本人小白,求相关细胞处理的过程及数据分析方法。贴壁细胞六孔板需要接种多少,染色后怎样计数进行数据处理。谢谢啦!
的抗体不容易染色,哪为同仁有这方面的经验?另外基因公司卖的cellsignalingtechnology的抗体要1875元,而中山公司卖的这个抗体只有800多,其中差距在哪里?请赐教
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