Recombinant Human ACE2-Fc-Avi Protein (rhACE2-Fc)
Source: recombinant human ACE2 protein (Gln18-Ser740) was expressed in mammalian cells with a Fc fusion and Avi at the C-terminus.Accession: Q9BYF1Predicted molecular mass: 111.2 kDa. Due to glycosylation, the rhACE2 protein migrates to 115-125 kDa based on the Bis-Tris PAGE result.Endotoxin: Less than 0.5 EU per ug by the LAL method.Activity: Immobilized biotinylated recombinant SARS-CoV-2 Spike S RBD with the His Tag at 1 ug/ml (100 ul/well). Dose response curve for rhACE2-Fc fusion protein with the EC50 of 81 ng/ml determined by ELISA. Conditions of optimal recombinant Human ACE2 protein (rhACE2-Fc) performance should be determined experimentally by the investigator.Formulation: The recombinant human ACE2-Fc-Avi protein was lyophilized from 0.22 um filtered solution in 20 mM PB (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.Purity: > 95% by PAGE under reduced condition, and SEC-HPLC.Shipping: The recombinant human ACE2-Fc-Avi protein is shipped with ice packs. Upon receipt, store it immediately at the temperature recommended below.Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.
Background
ACE2 (Angiotensin I Converting Enzyme 2) belongs to the angiotensin-converting enzyme family of dipeptidylcarboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome (SARS) including Coronavirus Disease 2019 (COVID-19), and Neurogenic Hypertension. The SARS-CoV and SARS-CoV-2 (2019-nCoV) spike (S) proteins mediate viral entry into host cells by binding to the ACE2 receptor through the receptor-binding domain (RBD) of the S1 subunit, and then fusing the viral and host membranes through the S2 subunit. The ACE2-binding affinity of the Spike RBD of SARS-CoV-2 is 10- to 20-fold higher than that of SARS-CoV. Two trimeric SARS-CoV-2 spike proteins bind to an ACE2 dimer with the collectrin-like domain of ACE2 mediating homodimerization.
The binding of the S1 subunit to the extracellular peptidase domain of ACE2 on the surface of cells results in endocytosis and translocation of both the virus and the enzyme into endosomes located within cells. This entry process also requires priming of the S protein by the host serine protease TMPRSS2, which is a potential drug for inhibition. The recombinant human ACE2 (rhACE2) may also be a promising drug for those with intolerance to classic renin-angiotensin system inhibitors (RAS inhibitors) or in diseases where circulating angiotensin II is elevated. Infused rhACE2 has been evaluated in clinical trials for the treatment of SARS.
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为充分发挥实验室和仪器设备的使用效益,同时,又保证试验的正常进行和仪器设备的完好率,如果你对实验室管理有任何好的建议,欢迎参与讨论,请不要吝啬你的语言,大家可以从以下话题发表一下自己的看法,谈谈你们实验室是如何进行管理的:
1,试验仪器如何使用和维护;
2,试验卫生如何进行清洁与维护;
3,实验室安全问题;
........
格式不限,有一计说一计,请不要在网上搜一堆相关的资料发帖参与讨论,更希望能看到你在实际管理中的经验交谈;
有疑问就有解答,同时也欢迎你提出你的疑问,期待你的参与!
荧光剂(又称增白剂、光学增白剂、荧光增白剂),这里提供一个简单低成本的方法检测,可以使用365nm的紫外线小电筒照射(也可以是紫外线验钞机),如有发蓝紫色荧光(一般为蓝、紫,也有绿、红,和原来的颜色对比发生变化,明显鲜艳了很多并发亮,多照照几个其他物品对比就知道了),则是含有荧光剂。这种电筒在TB上有卖,搜索(荧光剂检测),不贵一般10-30多块钱左右,还是比较实用的,纺织物、塑料制品、护肤品、纸张等(有的食品有特殊的荧光反应除外,比如大米的脂肪会有荧光反应)含有荧光剂都能照射出来。以后就注意检测一下,特别是宝宝用的,或是贴身用品、护肤品。如果是三无产品确实有含荧光剂的风险。
在日常生活中,接触荧光剂的机会很多。只要不超过一定标准(上述方法只能检测出是否含有荧光剂,不能检查出是否超标),会给我们生活带来不少好处。如果过量的与它接触,会对人体造成伤害。
提示:紫外线在不照射荧光物质下,是看不到的(如果有少量白光那是灯珠发出的部分可见光,并不是紫外线),如果反光能看到,说明被照射物体发生了荧光反应。紫外线对人体有害,避免直射人体和长时间使用。
不懂的可以继续问,望采纳,谢谢。
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