描述
Thequbit4FlurometeristhelatestversionofthepopularQubitfluorometerdesignedtoaccuratelymeasureDNA,RNA,andproteinquantity,andnowalsoRNAintegrityandquality,usingthehighlysensitiveQubitassays.TheQubit4Fluorometerwasre-engineeredtoruntheQubitRNAIQ(integrity&quality)assay.TheQubit4FluorometerandRNAIQAssayKitworktogethertoaccuratelydistinguishintactfromdegradedRNAinjusttwoeasysteps.
ForallQubitassays,theconcentrationorqualityofthetargetmoleculeinthesampleisreportedbyafluorescentdyethatemitsasignalonlywhenboundtothetarget,whichminimizestheeffectsofcontaminantsontheresult.Theeasy-to-usetouch-screenmenusmakeiteasytoselectandruntheassaysyouneed,withresultsdisplayedinjustafewseconds.
KeyfeaturesoftheQubit4Fluorometerinclude:
•FastandhighlyaccuratequantitationofDNA,RNA,andproteininlessthanthreesecondspersample
•MeasuresintactRNAinlessthan5secondspersample
•Highlevelsofaccuracyusingonly1–20μLofsample,evenwithverydilutesamples
•On-boardReagentCalculatorquicklygeneratesQubitworkingsolutionpreparationinstructions
•Storesresultsfromupto1000samples
•Large5.7-inch,state-of-the-artcolortouchscreenforeasyworkflownavigation
•Graphicaldisplayindicateswhensamplesareintheextendedrangeoroutofrange
•Smallfootprintsavesspaceonyourbench
•ExportsdatatoaUSBdriveordirectlytoyourcomputerviaaUSBcable
•ABIlitytopersonalizeyourQubitfluorometerwiththeassaysyourunmost,addnewassays,orevencreateyourownassayswiththeMyQubitsoftwareandwebtool
•LanguageofyourchoiceincludingEnglish,French,Spanish,Italian,German,simplifiedChinese,andJapanese
TheQubit4FlurometercanalsobeusedtodirectlymeasurethefluorescenceofsamplesusingFluorometermode.Additionally,IonSphereParticlequalitycanbeevaluatedontheQubit4FluorometerusingtheIonSphereQualityControlKitpriortoperformingasequencingrunontheIonPGMsequencer.
EnablesmoreaccurateresultsthanwithUVabsorbanceatlowconcentrations
TheQubit4FluorometerisfarmoresensitivethanUVabsorbance,whichmeasuresanythingabsorbingat260nm,includingDNA,RNA,protein,freenucleotides,orexcesssalts.Moreover,UVspectrophotometryisoftennotsensitiveenoughtoaccuratelymeasurelowconcentrationsofDNAandRNA.WiththeQubitfluorometer,yourresearchisenhancedbymoreaccuratemeasurementsbecausethedyesintheQubitassaykitsfluoresceonlywhenboundtotheselectedmolecule(DNA,RNAorprotein)inyoursample.Thisallowsyoutoavoidrepeatingworkduetoinaccuratemeasurements.
Fast,simple,andaccuratemeasurementofRNAquality
TheQubitRNAIQassayprovidesafast,simplemethodtocheckwhetheranRNAsamplehasdegraded.Theassayutilizestwouniquedyes:onethatbindstolarge,intactRNA,andanotherthatselectivelybindstosmall,degradedRNA.TogethertheyenableyoutoquicklyassessthequalityandintegrityofanRNAsample.Touse,simplyaddyoursamplestotheRNAIQworkingsolution,thenmeasureontheQubit4Fluorometer.
Savesamplesandbenchspace
ThethoughtfullydesignedQubit4Fluorometerrequiressamplesofonly1–20µL.Itisintendedforuseatroomtemperatureandoccupiesonlyasmallamountofbenchspace.TheQubit4Fluorometerfeaturesadvancedopticsanddataanalysisalgorithms,andcomesequippedwithaUSBthumbdriveandcablefordatatransfertoExcel™softwareandforsoftwaredownloads,aswellasauniversalpowersupply,fourplugadapters,andelectromagneticCEcertification.
Singlesamplemeasurementsareabreeze
Thisuser-friendlyfluorometeriscombinedwithquickandsimpleassayprocedures.Calculationsandsettingsareautomaticallyperformedbytheinstrument.TheQubitassaysforusewiththeQubit4Fluorometerareallperformedusingthesamegeneralprotocol,whichusesasimplemix-and-readformatwithincubationtimesofonlytwominutesfortheDNAandRNAassays.500Lthin-walledPCRtubes(Cat.No.Q32856)arerequired.
Systemspecificationsinclude:
Instrumentdimensions: 5.4"(w)x10"(l)x2.2"(h)(13.6cmx25cmx5.5cm)
Weight: 743g
Dynamicrange: 5ordersofmagnitude
Processingtime: ≤seconds/sample
Lightsources: blueLED(max~470nm),redLED(max~635nm)
Excitationfilters: blue(430–495nm),red(600–645nm)
Emissionfilters: green(510–580nm),red(665–720nm)
Detectors: photodiodes,measurementcapabilityfrom300–1,000nm
Warm-uptime: <35seconds
USBthumbdrive: 4GB
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为充分发挥实验室和仪器设备的使用效益,同时,又保证试验的正常进行和仪器设备的完好率,如果你对实验室管理有任何好的建议,欢迎参与讨论,请不要吝啬你的语言,大家可以从以下话题发表一下自己的看法,谈谈你们实验室是如何进行管理的:
1,试验仪器如何使用和维护;
2,试验卫生如何进行清洁与维护;
3,实验室安全问题;
........
格式不限,有一计说一计,请不要在网上搜一堆相关的资料发帖参与讨论,更希望能看到你在实际管理中的经验交谈;
有疑问就有解答,同时也欢迎你提出你的疑问,期待你的参与!
1.两种方法哪一种更加准确?
2.那一种方法更方便?
3.需要的仪器上海哪家单位有?
但是因为荧光剂是一种会溶于液体并随之迁移又很难去除的化学物质,所以一旦洗衣液中的荧光剂附着在衣物上,用洗衣机大力清洗,也不用担心被洗掉,对人的皮肤和身体都有害,所以选择不含荧光剂的衣衣不舍洗衣宝是必要的。
人误服后,如果出现胸痛、恶心、呕吐、腹泻、头晕和便血等不良反应,应尽快予以催吐,可用手指或羽毛之类的物品,刺激其咽喉部,使其产生恶心感。在催吐后可内服牛奶、鸡蛋清、豆浆、稠米汤和花生油等,并及时送医院急救处理。
误喝了少量荧光剂,医院要给他洗胃、灌肠,用一些保护肠胃黏膜的药。同时,在一两天内尽量不要进食,静养几天,就会痊愈的。
荧光剂对人体的危害:
荧光剂不像一般化学成分那样容易被分解,而是在人体内蓄积,产生许多有害的作用,大大削减人体免疫力;荧光剂与伤口外的蛋白质结合,还会阻碍伤口的愈合;荧光剂能使人体细胞出现变异性倾向,其毒性累积在肝脏或其他重要器官,会成为潜在的致癌因素。造成血液系统受损:
化学物质容易污染人体血液,虽然血液具有一定的自净能力,微量的有害物质进入其中,会被稀释、分解、吸附和排出,但长期、大量的有毒物质倾注而入,必致其发生质的变化;进入血液循环,会破坏红细胞的细胞膜,引起溶血现象。
市面上的一些荧光剂,为了使之能够长久发光,部分不法分子会加入一些放射性物质,放射性物质的射线被荧光物质吸收从而发光,放射性物质显然是容易致癌的,某些放射性物质进入身体还有可能会变成内放射源。
但无论如何,荧光物质种类众多,不能一概而论。
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