单克隆抗体是由单一B细胞克隆产生的高度均一、仅针对某一特定抗原表位的抗体，称为单克隆抗体。通常采用杂交瘤技术来制备，杂交瘤（hybridoma）抗体技术是在细胞融合技术的基础上，将具有分泌特异性抗体能力的致敏B细胞和具有无限繁殖能力的骨髓瘤细胞融合 查看更多>

是指免疫系统中的免疫细胞和免疫分子之间，以及与其它系统如神经内分泌系统之间的相互作用，使得免疫应答以最恰当的形式维持在最适当的水平。免疫调节可比喻为机体的交响乐队，配合好--识别和清除抗原，对自身成分产生免疫耐受，维持内环境的稳定。配合 查看更多>

MicroinjectionDNAPurificationWehavefoundtheNucleoSpinTMExtractKit(availablefromClontech,CatalognumberK3051-1)isasimpleandfastwaytoobtainmicroinjectionqualityDNA.ThisisnottoexcludeothermethodsofDNApurificationbuttosaythatwefindthisisaconvenientmethodthatislessonerousthanCsClgrADIents.1.Performrestrictiondigesttoliberatetransgenefromplasmidvectorsequ 查看更多>

AlkalineLysisandCsClPurificationofBACDNAforTransgenicProduction.ThismethodwascontributedbyMichelleSouthard-SmithandRonChandleratVanderbiltUniversity,Nashville,Tennessee.ThisprocedurehasbeenderivedfromacombinationofmethodsusedtoisolatehighMWDNAs.ThelysisconditionsandCsClstepswereincorporatedfrommethodsusedinRayMacDonald’slabatUTSouthwesternMedical 查看更多>

ThismethodwasestablishedintheUniversityofMichiganTransgenicCoreforourusers.ThemethodisusedtopurifyBACDNAformicroinjectionintofertilizedmouseeggsandBACtransgenicmouseproduction..Thefollowingmethodyieldsclean,non-shearedDNA.SomeofinformationbelowwastakenfromTheNestGroupwebsite(TheNestGroup,Inc.TEL800-347-6378,FAX508-485-5736).Materials:Thekitsarenolo 查看更多>

BACDNAhasbeenpurifiedsuccessfullyformicroinjectionbythreeprinciplemethods:AnionExchangeChromatography(NucleobondColumns),CsClGrADIent,orSepharose4B-CLChromatography.AnyofthesemethodscanbeusedsuccessfullytopurifyBACDNAforpronuclearmicroinjectionwhentheprotocolsarefollowedcarefully.Inourhands,successfulBACpreparationsrequirethepolyaminemicroinjection 查看更多>

WholemountinsituhybridisationBasedonWilkinsonprotocol,modifiedbyMurrayHargrave(m.hargrave@cmcb.uq.edu.au),KoopmanlabA.SYNTHESISOFPROBE1Mixinthefollowingorderatroomtemperature:steriledistilledwater9.5µL5xtranscriptionbuffer4µL0.2MDTT1µLnucleotidemix(pH8.0)2µL(10xDIGRNAlabellingmix)linearisedplasmid(1µg/µL)1µ 查看更多>

imageofawholemountfroma4wkoldvirginProtocolSpreadtissueonglassslideFixinCarnoy"sfixativefor2to4hoursatr.t.Washin70%EtOHfor15minChangegraduallytodistilledwaterRinseindistilledwaterfor5minStainincarminealumO/NWashin70%EtOH15minWashin95%EtOH15minWashin100%EtOH15minClearinxyleneandmountwithPermountAfterphotographicdocumentationthetissuecanbeembeddedinp 查看更多>

DNAPreparationformicroinjectionorelectroporationPronuclearinjectiontoproducetransgenicmiceMorulaAggregationtoproducechimericmiceTransgenicidentificationPCRofDNAfromearcliptissueGenomicSouthernsofDNAfromtailclipsAnimalProtocolsObtainingaUniversityapprovedanimalprotocolMatingtransgenicmiceDNAPreparationforMicroinjectionorElectroporationThisprotocolwa 查看更多>

Analgesicandanestheticdrugsmayhaveanimpactontheresultsachievedfromanimalexperiments.Inthestudypresentedhere,wetrytoenlightenwhetheranesthesiawithfentanyl/fluniasoneandmidazolam(HypnormandDormicum)hasaninfluenceonmeasurementsofhemoglobininmice.Inacross-overstudy,wehavecomparedhemoglobinlevelsintwogroupsofmice:anesthetizedversusnon-anesthetizedandfou 查看更多>

羊抗小鼠IgG-RBITCCDR移植即把鼠抗体的CDR序列移植到人抗体的可变区内，所得到的抗体称CDR移植抗体或改型抗体，也就是人源化抗体。全合成法是以人抗体序列为骨架，以鼠抗体的CDR置换人抗体的CDR，将整个可变区序列的两条链分解成若干片段，并使相邻的片段具有� 查看更多>

兔抗小鼠IgG-FC人一鼠嵌合抗体与鼠单抗相比，免疫原性大大降低，利于在人体内应用，所以目前已制备出上百种抗各种抗原（包括肿瘤相关抗原）的嵌合抗体。尽管嵌合抗体的免疫原性已降低很多，但有时它仍可能引发较强的免疫反应。为了进一步降低抗体的鼠源� 查看更多>