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单克隆抗体的制备过程详解2017-07-11
单克隆抗体是由单一B细胞克隆产生的高度均一、仅针对某一特定抗原表位的抗体,称为单克隆抗体。通常采用杂交瘤技术来制备,杂交瘤(hybridoma)抗体技术是在细胞融合技术的基础上,将具有分泌特异性抗体能力的致敏B细胞和具有无限繁殖能力的骨髓瘤细胞融合 查看更多
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蚂蚁淘带你了解:免疫调节2016-10-23
是指免疫系统中的免疫细胞和免疫分子之间,以及与其它系统如神经内分泌系统之间的相互作用,使得免疫应答以最恰当的形式维持在最适当的水平。免疫调节可比喻为机体的交响乐队,配合好--识别和清除抗原,对自身成分产生免疫耐受,维持内环境的稳定。配合 查看更多
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Microinjection DNA Purification2018-12-26
MicroinjectionDNAPurificationWehavefoundtheNucleoSpinTMExtractKit(availablefromClontech,CatalognumberK3051-1)isasimpleandfastwaytoobtainmicroinjectionqualityDNA.ThisisnottoexcludeothermethodsofDNApurificationbuttosaythatwefindthisisaconvenientmethodthatislessonerousthanCsClgrADIents.1.Performrestrictiondigesttoliberatetransgenefromplasmidvectorsequ 查看更多
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AlkalineLysisandCsClPurificationofBACDNAforTransgenicProduction.ThismethodwascontributedbyMichelleSouthard-SmithandRonChandleratVanderbiltUniversity,Nashville,Tennessee.ThisprocedurehasbeenderivedfromacombinationofmethodsusedtoisolatehighMWDNAs.ThelysisconditionsandCsClstepswereincorporatedfrommethodsusedinRayMacDonald’slabatUTSouthwesternMedical 查看更多
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ThismethodwasestablishedintheUniversityofMichiganTransgenicCoreforourusers.ThemethodisusedtopurifyBACDNAformicroinjectionintofertilizedmouseeggsandBACtransgenicmouseproduction..Thefollowingmethodyieldsclean,non-shearedDNA.SomeofinformationbelowwastakenfromTheNestGroupwebsite(TheNestGroup,Inc.TEL800-347-6378,FAX508-485-5736).Materials:Thekitsarenolo 查看更多
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BACDNAhasbeenpurifiedsuccessfullyformicroinjectionbythreeprinciplemethods:AnionExchangeChromatography(NucleobondColumns),CsClGrADIent,orSepharose4B-CLChromatography.AnyofthesemethodscanbeusedsuccessfullytopurifyBACDNAforpronuclearmicroinjectionwhentheprotocolsarefollowedcarefully.Inourhands,successfulBACpreparationsrequirethepolyaminemicroinjection 查看更多
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Wholemount in situ hybridisation2018-12-26
WholemountinsituhybridisationBasedonWilkinsonprotocol,modifiedbyMurrayHargrave(m.hargrave@cmcb.uq.edu.au),KoopmanlabA.SYNTHESISOFPROBE1Mixinthefollowingorderatroomtemperature:steriledistilledwater9.5µL5xtranscriptionbuffer4µL0.2MDTT1µLnucleotidemix(pH8.0)2µL(10xDIGRNAlabellingmix)linearisedplasmid(1µg/µL)1µ 查看更多
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whole mount preparations2018-12-26
imageofawholemountfroma4wkoldvirginProtocolSpreadtissueonglassslideFixinCarnoy"sfixativefor2to4hoursatr.t.Washin70%EtOHfor15minChangegraduallytodistilledwaterRinseindistilledwaterfor5minStainincarminealumO/NWashin70%EtOH15minWashin95%EtOH15minWashin100%EtOH15minClearinxyleneandmountwithPermountAfterphotographicdocumentationthetissuecanbeembeddedinp 查看更多
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Transgenic and Knockout Mice PROTOCOL2018-12-26
DNAPreparationformicroinjectionorelectroporationPronuclearinjectiontoproducetransgenicmiceMorulaAggregationtoproducechimericmiceTransgenicidentificationPCRofDNAfromearcliptissueGenomicSouthernsofDNAfromtailclipsAnimalProtocolsObtainingaUniversityapprovedanimalprotocolMatingtransgenicmiceDNAPreparationforMicroinjectionorElectroporationThisprotocolwa 查看更多
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Analgesicandanestheticdrugsmayhaveanimpactontheresultsachievedfromanimalexperiments.Inthestudypresentedhere,wetrytoenlightenwhetheranesthesiawithfentanyl/fluniasoneandmidazolam(HypnormandDormicum)hasaninfluenceonmeasurementsofhemoglobininmice.Inacross-overstudy,wehavecomparedhemoglobinlevelsintwogroupsofmice:anesthetizedversusnon-anesthetizedandfou 查看更多
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羊抗小鼠IgG-RBITC2018-05-30
羊抗小鼠IgG-RBITCCDR移植即把鼠抗体的CDR序列移植到人抗体的可变区内,所得到的抗体称CDR移植抗体或改型抗体,也就是人源化抗体。全合成法是以人抗体序列为骨架,以鼠抗体的CDR置换人抗体的CDR,将整个可变区序列的两条链分解成若干片段,并使相邻的片段具有 查看更多
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兔抗小鼠IgG-FC2018-05-25
兔抗小鼠IgG-FC人一鼠嵌合抗体与鼠单抗相比,免疫原性大大降低,利于在人体内应用,所以目前已制备出上百种抗各种抗原(包括肿瘤相关抗原)的嵌合抗体。尽管嵌合抗体的免疫原性已降低很多,但有时它仍可能引发较强的免疫反应。为了进一步降低抗体的鼠源 查看更多
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