相关文章
>
WholeIgGantibodiesareisolatedasintactmoleculesfromantiserabyimmunoaffinitychromatography.TheyhaveanFcportionandtwoantigenbindingFabportionsjoinedtogetherbydisulfidebondsandthereforetheyaredivalent.Theaveragemolecularweightisreportedtobeabout160kDa.ThewholeIgGformofantibodiesissuitableforthemajorityofimmunodetectionproceduresandisthemostcosteffective.
Basedonimmunoelectrophoresisand/orELISA,theantibodyreactswithwholemoleculerabbitIgG.Italsoreactswiththelightchainsofotherrabbitimmunoglobulins.Noantibodywasdetectedagainstnon-immunoglobulinserumproteins.Theantibodymaycross-reactwithimmunoglobulinsfromotherspecies.
PhysicalState:Freeze-driedsolid
Storage:
Storefreeze-driedpowderat2-8°C.Whenreadytouse,rehydratewithindicatedvolumeofd.waterandcentrifugeifnotclear.Productisstableforabout6weeksat2-8°Casanundilutedliquid.Prepareworkingdilutionfresheachday.Forextendedstorageafterrehydration,addanequalvolumeofglycerol(ACSgradeorbetter)forafinalconcentrationof50%,andstoreat-20°Casaliquid.Note:aftertheadditionofglycerol,theconcentrationofproteinandbuffersaltsisone-halfoftheoriginal.Alternatively,aliquotandfreezetheproductat-70°Corbelowintheabsenceofglycerol.Avoidrepeatedfreezingandthawing.
Expirationdate:oneyearfromdateofrehydration.However,theexpirationdatemaybeextendediftheproductisstoredaccordingtotherecommendationandthetestresultsareacceptableforitsintendeduse.
Purity:Theantibodywaspurifiedfromantiserabyimmunoaffinitychromatographyusingantigenscoupledtoagarosebeads.
Buffer:0.01MSodiumPhosphate,0.25MNaCl,pH7.6
StABIlizer:15mg/mlBovineSerumAlbumin(IgG-Free,Protease-Free)
Preservative:None(Warning:UseofsodiumazideasapreservativewillsubstantiallyinhibittheenzymeactivityofhorserADIshperoxidase.)
SuggestedWorkingConcentrationorDilutionRange:
ELISA:-1:5,000-1:100,000
WesternBlot:-1:5,000-1:100,000(non-ECL)/1:10,000-1:200,000(ECL)
Histo-/Cyto-Chemistry:-1:500-1:5,000
Dilutionfactorsarepresentedintheformofarangebecausetheoptimaldilutionisafunctionofmanyfactors,suchasantigendensity,permeability,etc.Theactualdilutionusedmustbedeterminedempirically.
Horseradishperoxidase(HRP)conjugatesarepreparedbyamodifiedNakaneandKawaoiprocedure(J.Histochem.Cytochem.1974.22,1084).Peroxidaseconjugatesarecommonlyusedforimmunohistochemistry,Westernblotting,andELISA.Affinity-purifiedanti-horseradishperoxidaseandconjugatesareavailablefordetectionofhorseradishperoxidaseantigenorforsignalamplificationofHRP-containingreagents.Forimmunostainingofmammaliancells,anadvantageofusinganti-horseradishperoxidaseisreducedbackground,sincetheantibodydoesnotrecognizetheendogenousperoxidase-likeenzymesfoundinthosecells.
蚂蚁淘所有产品都是自运营的，我们已经跟国外多家厂方建立品牌推广合作关系, 获得对方的支持和授权； 同时客户可以通过订单详情查看到货物从厂方至客户的所有流程， 确保货物的来源； 正规报关，提供13%增值税发票。
蚂蚁淘的运营团队都是有着多年经验的成员，他们熟悉海外采购、仓储物流、报关等环节； 同时通过在线的流程监控，蚂蚁淘的进口速度比传统企业提高了50%以上， 部分产品甚至能做到7-10天到货，即蚂蚁淘的“时必达”服务。
蚂蚁淘的价格是真实透明的，并且具有很大的价格优势，不需要繁杂的询价比价； 报价单与合同可以直接在线生成或打印；就像在京东购物一样， 您的鼠标点击几 次即完成在蚂蚁淘的采购，订单详情会告诉您所有进程。
蚂蚁淘提供的产品在使用过程中如因产品质量问题有售后需求时， 您可通过我的订单提交您的“申请售后”， 蚂蚁淘产品顾问会第一时间为您处理， 在售后服务过程中如遇到问题也可致电蚂蚁淘客服热线：4000-520-616。
版权所有 : ©2005-2020 苏州蚂蚁淘生物科技有限公司 苏CP备17049038号
公司邮箱 : info@ebiomall.com
联系电话 : 4000-520-616
© 2005-2020 苏州蚂蚁淘生物科技有限公司 版权所有。 中国
Tel: 4000-520-616
E-mail: info@ebiomall.com
苏ICP备17049038号
苏公网安备 32059002001627号
