Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)TTKINMDDLQPSENEDKS,correspondingtoaminoacid residues848-865ofratCaV1.2(Accession P22002).IntracellularloopbetweendomainsIIandIII.
- Westernblotanalysisofratbrainmembranes:1. Anti-CaV1.2(CACNA1C)Antibody(#ACC-003),(1:200).
2.Anti-CaV1.2(CACNA1C)Antibody,preincubatedwiththecontrolpeptideantigen.WesternblotanalysisofCaV1.2-transfected Xenopus oocytes(lane1)andnon-transfectedoocyteslysates(lane2):1. Anti-CaV1.2 (CACNA1C) Antibody(#ACC-003),(1:200)in CaV1.2(CACNA1C)ChannelOverexpressedMembraneFractions(LX-104).
2.Anti-CaV1.2(CACNA1C)Antibodyinnon-transfectedoocytes.Humancardiactissue(Crossman,D.J. etal. (2011) PLoSONE 6, e17901.).
- CaV1.2transfectedHEK-293cellsandmouseheartlysate(Rougier,J.S. etal. (2011) J.Biol.Chem. 286, 8829.).
- ExpressionofCaV1.2inmousecerebellumImmunohistochemicalstainingofmousecerebellumwith Anti-CaV1.2 (CACNA1C) Antibody(#ACC-003).A.CaV1.2(red)appearsinPurkinjecells(horizontalarrows)andisdistributeddiffuselyinthemolecularlayer(Mol)includinginPurkinjedendrites(verticalarrows).B.StainingofPurkinjenervecellswithmouseanti-Calbindin28K (green)demonstratesthelocationofdendritesinthemolecularlayer.C.MergedimageofpanelsAandB.Mouseatrioles(1:300)(Howitt,L. etal. (2013) J.Physiol. 591, 2157.).
- Ratinsulinomacells(RIN)(Parkash,J.(2011) LifeSci. 88, 257.).Mousechromaffincells(1:200).(Perez-Alvarez,A. etal. (2011) J.NeuRochem. 116, 105.).
- Ratmastcells(RBL-2H3cells,1μg/5x105 cells).(Yoshimaru,T. etal. (2009) Mol.Immunol. 46, 1267.).Thecontrolantigenisnotsuitableforthisapplication.
- 1.Catterall,W.A.etal.(2003)Pharmacol.Rev.55,579.
- 2.IUPHAR
- 3.Hu,X.Q.etal.(1998)J.Biol.Chem.273,5337.
- 4.Kreuzberg,U.etal.(2000)AmJ.Physiol.278,H723.
- 5.Allard,B.etal.(2000)J.Biol.Chem.275.25556.
AllL-typecalciumchannelsareencodedbyoneoftheCaV1channelgenes.ThesechannelsplayamajorroleasaCa2+ entrypathwayinskeletal,cardiacandsmoothmusclesaswellasinneurons,endocrinecellsandpossIBLyinnon-excitablecellssuchashematopoeticandepithelialcells.AllCaV1channelsareinfluencedbydihydropyridines(DHP)andarealsoreferredtoasDHPreceptors.
WhiletheCaV1.1andCaV1.4isoformsareexpressedinrestrictedtissues(skeletalmuscleandretina,respectively),theexpressionofCaV1.2isubiquitousand CaV1.3 channelsarefoundintheheart,brainandpancreas.SeveralpeptidyltoxinsaredescribedthatarespecificL-typechannelblockers,butsofarnoselectiveblockerforoneoftheCaV1isoformshavebeendescribed.TheseincludetheMambatoxins Calcicludine (#SPC-650), Calciseptine (#C-500)and FS-2 (#F-700).
ExpressionofCaV1.2inratcardiomyocytes.ImmunocytochemicalstainingofadultratcardiomyocytesusingAnti-CaV1.2(CACNA1C) Antibody(#ACC-003).CaV1.2staining(green)isobservedinaregularlyspacedarray,aswellassurfacesarcolemmalstaining.β-arrestinstainingisinred.AdaptedfromHermosilla,T.etal.(2017)Sci.Rep.7,10131.withpermissionofSPRINGERNATURE.
Anti-CaV1.2(CACNA1C)Antibody(#ACC-003)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunoprecipitation,immunohistochemistry,immunocytochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeCaV1.2frommouse,rat,andhumansamples.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
抗人球蛋白试验,又称Coombs’试验,是诊断自身免疫性溶血性贫血(AIHA)的重要依据。
正常人直接与间接试验均为阴性,Coombs’试验阴性有时并不能排除AIHA
本试验阳性结果主要见于下列几种情况:
1.自身免疫性贫血,(IgG)型引起的溶血性贫血,本试验直接反应常呈强阳性,间接反应大多阴性,但亦可阳性。
2.药物诱发的免疫性溶血性贫血①α-甲基多巴型:直接及间接反应均阳性。②青霉素型:直接反应阳性,间接反应阴性,以上二型如以正常红细胞先与有关药物于37℃培育后再加病人血清、间接反应均为阳性。③福阿亭型:(奎宁等药物)抗体通常为IgM,偶有IgG型者,直接反应为阳性,间接反应阴性,但如用IgG抗血清做试剂则结果大部分均为阴性,但如培育时加入新鲜正常人血清(供应补体)则结果为阳性。
3.冷凝集素综合征直接反应阳性,间接反应阴性(试验需在37℃下进行)由于本病红细胞膜附着的是补体C4和C3而不是IgG或IgM,如果用抗IgG或抗IgM抗血清做试验时,则结果阴性,如以抗补体的抗血清做试验则直接反应阳性。
4.新生儿同种免疫溶血病,因Rh血型不合所致溶血病,直接及间接反应均强阳性,持续数周、换血输血后数天内可变为阴性,由于“ABO”血型不合引起的溶血病,结果常为阴性或弱阳性。
5.红细胞血型不合引起的输血反应,ABO或Rh血型不合输血,供者的红细胞被受者的血型抗体致敏,在供者被致敏的红细胞完全破坏以前,直接反应阳性,Rh阴性者如过去不曾接受过Rh阳性者的血或曾妊娠胎儿为Rh阳性者,间接反应阳性,如无上述接触,第一次输血后(Rh阳性的血),数天之内间接反应也会变为阳性。
6.其它在传染性单核细胞增多症、SLE、恶性淋巴瘤、慢性淋巴细胞白血病、癌肿、铅中毒、结节性动脉周围炎、EVan氏综合征等,病人直接反应亦可阳性,阵发性寒冷性血红蛋白尿症患者中,急性发作后用抗补体血清做试验直接反应常为阳性。展开
鼠抗人CD3单克隆抗体针对人的CD3
CD3单克隆抗体,要看其说明书,该抗体是否识别其他生物(如大鼠)的CD3.
暂无品牌问答