This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
- Peptide (C)RIGLLGHPPHMNVNPQQPA, corresponding to amino acid residues 2732-2750 of rat IP3R1 (Accession P29994). Intracellular, C-terminus.
- Western blot analysis of rat brain membranes:1. Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019), (1:2000).
2. Anti-IP3 Receptor-1 (ITPR1) Antibody, preincubated with the control peptide antigen.
- Rat superior cervical ganglia (SGC) lysate (Zhang, H. et al. (2013) Neuroscience 254, 70.).
- Expression of IP3R1 in mouse cerebellumImmunohistochemical staining of mouse cerebellum with Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019). Immunoreactivity appears in Purkinje cells (arrows in A) and their dendritic trees. In addition, axonal processes coursing through cerebellar white matter are visualized (arrow in B).
- 1. Blondel, O. et al. (1993) J. Biol. Chem. 268, 11356.
- 2. V"arnai, P. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 7859.
- 3. Cai, W. et al. (2004) J. Biol. Chem. 279, 23691.
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Inositol 1,4,5-triphosphate (IP3) serves as a second messenger for many neurotransmitters, hormones and growth factors.1 The binding of IP3 to its receptor (IP3R), which is a ligand-gated Ca2+ channel, located predominantly at the endoplasmic reticulum, results in a rapid release of Ca2+ from intracellular stores.1
To date, three known isoforms of IP3R are known (designated IP3R1, IP3R2, and IP3R3) that can work as homotetramers or hetrotetramers. All three receptors have six transmembrane domains and a pore region between TM5 and TM6. The N-terminus as well as the C-terminus face the cytoplasm. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required in order to maintain the suppression of channel activity.2
IP3R1 is one of the most important channels responsible for Ca2+ release from intracellular stores and was shown to be the predominant isoform expressed in the central nervous system. IP3R1 is a pivotal player in many neuronal functions, amongst them, neuronal plasticity and neurite extension.3
Expression of IP3 receptor 1 in TA muscle sections.Immunohistochemical staining of mouse muscle sections using Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019). IP3R1 staining (green) is detected at the neuromuscular junction (upper panel). Staining is almost completely eradicated upon siRNA treatment (middle panel). IP3R1 specific staining is shown upon scrambled RNA (ctRNA) treatment (lower panel).Adapted from Zhu, H. et al. (2011) J. Neurosci. 31, 15269. with permission of the Society for Neuroscience.
Anti-IP3 Receptor-1 (ITPR1) Antibody (#ACC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize IP3R1 from human, rat, and mouse samples.
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多谢!
不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!
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