Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)TTKINMDDLQPSENEDKS,correspondingtoaminoacid residues848-865ofratCaV1.2(Accession P22002).IntracellularloopbetweendomainsIIandIII.
- Westernblotanalysisofratbrainmembranes:1. Anti-CaV1.2(CACNA1C)Antibody(#ACC-003),(1:200).
2.Anti-CaV1.2(CACNA1C)Antibody,preincubatedwiththecontrolpeptideantigen.WesternblotanalysisofCaV1.2-transfected Xenopus oocytes(lane1)andnon-transfectedoocyteslysates(lane2):1. Anti-CaV1.2 (CACNA1C) Antibody(#ACC-003),(1:200)in CaV1.2(CACNA1C)ChannelOverexpressedMembraneFractions(LX-104).
2.Anti-CaV1.2(CACNA1C)Antibodyinnon-transfectedoocytes.Humancardiactissue(Crossman,D.J. etal. (2011) PLoSONE 6, e17901.).
- CaV1.2transfectedHEK-293cellsandmouseheartlysate(Rougier,J.S. etal. (2011) J.Biol.Chem. 286, 8829.).
- ExpressionofCaV1.2inmousecerebellumImmunohistochemicalstainingofmousecerebellumwith Anti-CaV1.2 (CACNA1C) Antibody(#ACC-003).A.CaV1.2(red)appearsinPurkinjecells(horizontalarrows)andisdistributeddiffuselyinthemolecularlayer(Mol)includinginPurkinjedendrites(verticalarrows).B.StainingofPurkinjenervecellswithmouseanti-Calbindin28K (green)demonstratesthelocationofdendritesinthemolecularlayer.C.MergedimageofpanelsAandB.Mouseatrioles(1:300)(Howitt,L. etal. (2013) J.Physiol. 591, 2157.).
- Ratinsulinomacells(RIN)(Parkash,J.(2011) LifeSci. 88, 257.).Mousechromaffincells(1:200).(Perez-Alvarez,A. etal. (2011) J.NeuRochem. 116, 105.).
- Ratmastcells(RBL-2H3cells,1μg/5x105 cells).(Yoshimaru,T. etal. (2009) Mol.Immunol. 46, 1267.).Thecontrolantigenisnotsuitableforthisapplication.
- 1.Catterall,W.A.etal.(2003)Pharmacol.Rev.55,579.
- 2.IUPHAR
- 3.Hu,X.Q.etal.(1998)J.Biol.Chem.273,5337.
- 4.Kreuzberg,U.etal.(2000)AmJ.Physiol.278,H723.
- 5.Allard,B.etal.(2000)J.Biol.Chem.275.25556.
AllL-typecalciumchannelsareencodedbyoneoftheCaV1channelgenes.ThesechannelsplayamajorroleasaCa2+ entrypathwayinskeletal,cardiacandsmoothmusclesaswellasinneurons,endocrinecellsandpossIBLyinnon-excitablecellssuchashematopoeticandepithelialcells.AllCaV1channelsareinfluencedbydihydropyridines(DHP)andarealsoreferredtoasDHPreceptors.
WhiletheCaV1.1andCaV1.4isoformsareexpressedinrestrictedtissues(skeletalmuscleandretina,respectively),theexpressionofCaV1.2isubiquitousand CaV1.3 channelsarefoundintheheart,brainandpancreas.SeveralpeptidyltoxinsaredescribedthatarespecificL-typechannelblockers,butsofarnoselectiveblockerforoneoftheCaV1isoformshavebeendescribed.TheseincludetheMambatoxins Calcicludine (#SPC-650), Calciseptine (#C-500)and FS-2 (#F-700).
ExpressionofCaV1.2inratcardiomyocytes.ImmunocytochemicalstainingofadultratcardiomyocytesusingAnti-CaV1.2(CACNA1C) Antibody(#ACC-003).CaV1.2staining(green)isobservedinaregularlyspacedarray,aswellassurfacesarcolemmalstaining.β-arrestinstainingisinred.AdaptedfromHermosilla,T.etal.(2017)Sci.Rep.7,10131.withpermissionofSPRINGERNATURE.
Anti-CaV1.2(CACNA1C)Antibody(#ACC-003)isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblot,immunoprecipitation,immunohistochemistry,immunocytochemistry,andindirectflowcytometryapplications.IthasbeendesignedtorecognizeCaV1.2frommouse,rat,andhumansamples.
ebiomall.com
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多谢!
不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!
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