Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
ThisantibodyisshippedwithitsantigenFREEofcharge!
- Peptide(C)TTKINMDDLQPSENEDKS,correspondingtoaminoacidresidues848-865ofratCaV1.2(Accession P22002).IntracellularloopbetweendomainsIIandIII.
- Westernblotanalysisofratbrainmembrane:1. Guineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001),(1:200).
2.GuineapigAnti-CaV1.2(CACNA1C)Antibody,preincubatedwiththenegativecontrolantigen.WesternblotanalysisofCaV1.2-transfected Xenopus oocytes(lane1)andnon-transfectedoocyteslysates(lane2):1. Guineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001),(1:200)in CaV1.2(CACNA1C)ChannelOverexpressedMembraneFractions(LX-104).
2.GuineapigAnti-CaV1.2(CACNA1C)Antibodyinnon-transfectedoocytes.
- Immuno-colocalizationofCaV1.2andGABA(A) α1ReceptorinratcerebellumImmunohistochemicalstainingofratcerebellumusingGuineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001)and Anti-GABA(A) α1Receptor(extracellular)-ATTO-488Antibody (#AGA-001-AG). A.CaV1.2(red)isdetectedmostlyinPurkinjecells(arrow).B.Inthesamesection, GABA(A)α1Receptor(green)isobservedinthegranulelayer.C.MergeofthetwoimagessuggestssomecolocalizationbetweenCaV1.2andGABA(A)α1ReceptorintheratgranulelayerbutonlyCaV1.2appearsinPurkinjecells.ExpressionofCaV1.2inhumanatriaImmunohistochemicalstainingofhumanleftatriumusingGuineapigAnti-CaV1.2 (CACNA1C) Antibody(#AGP-001),(1:100).
ThepicturewaskindlyprovidedbyDr.VanWagoner,D.R.fromtheDepartmentofMolecularCardiology,ClevelandClinic,Cleveland,Ohio,USA.Lovano,B.andPeterson,J.collectedthedata.ExpressionofCaV1.2inratheartImmunohistochemicalstainingofratheartparaffinembeddedsectionsusingGuineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001).A.CaV1.2staining(green)appearsmainlyinthecardiacmuscle,andinalesserintensityinthetunicaintimalayerofthesmoothmuscleofthemusculararteries.B.NuclearstainingusingDAPIasthecounterstain.C.MergedimagesofAandB.ExpressionofCaV1.2inmousehippocampusImmunohistochemicalstainingofmousedentategyrususing Guineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001).A.CaV1.2(green)appearedintheoutermolecularlayerofthedentategyrusandinthegranulelayer.B.CounterstainwithDAPI(blue)outlinesthegranulelayerofthedentategyrus.
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Voltage-gatedCa2+ channels(CaV),enablethepassageofCa2+ ionsinavoltagedependentmanner.Theseheteromericentitiesareformedinpartbythepore-forming α1subunitwhichdeterminesthebiophysicalandpharmacologicalpropertiesofthechannel1.
L-typeCa2+ channelsmakeuponeofthreevoltage-gatedCa2+ channelfamilies.Fourdifferent α1isoforms(CaV1.1toCaV1.4)belongtotheL-typesubfamily.Structurally,each α1subunithasfourhomologousdomains(I-IV)andeachdomainhasasixtransmembranesection.Likemanyothervoltage-gatedchannels,L-typeCa2+ channelshaveauxiliarysubunitswhichareresponsIBLeformodulatingthesurfaceexpressionandpropertiesofthechannels2-5.
CaV1.1ismostlyexpressedintheskeletalmuscle,whileCaV1.4ismainlydetectedintheretina.TheexpressionofbothCaV1.2and CaV1.3 ismoreextensiveandincludesneurons,heart,smoothmuscle,innerear,retinaandpancreas6.L-typeCa2+ channelsareinvolvedinandmodulateavarietyofphysiologicalfunctionssuchasmusclecontraction,hormonesecretion,neuronalexcitABIlityandgeneexpression5.
CaV1.2undergoesvariouspost-translationalmodifications.Forexample,itcanundergoproteolyticcleavageatitsC-terminal.Thiscleavagehasbeenshowntotakeplaceinneuronsfollowingtheactivationof NMDA receptors5,7 andintheheart5,8,9.Thecleavedmoietycanstillinteractwiththechannelanditsgeneralpurposeistomodulatechannelactivity5.OtherpostranslationmodificationsofthechannelincludephosphorylationofCaV1.2byanumberofkinasessuchasPKA,PKC,SrcandCaMKII5.Inaddition,itisnotsurprisingthatphosphatasesalsoregulatechannelactivity,astheyarerequiredtoantagonizetheactivityofthevariouskinasesknowntophosphorylateCaV1.2 5.
ThefactthatCaV1.2playsaprominentroleinpropercardiacfunctionhaspromptedendlessstudiesregardingitsregulation.Suchstudieshaveconcludedthatdysregulationofthechannelleadstoanomaliesinheartcontractionandthusheartfailure5.Likewise,CaV1.2defectshavebeendetectedinautismandbipolardisorder10.
Immuno-colocalizationofCaV1.2andGABA(A) α1ReceptorinratandmousehippocampusImmunohistochemicalstainingofmouseandrathippocampaldentategyrususingGuineapig Anti-CaV1.2 (CACNA1C) Antibody(#AGP-001)and Anti-GABA(A)α1Receptor(extracellular)-ATTO-488Antibody (#AGA-001-AG)inthesamesection.BothCaV1.2(red)andGABA(A)α1Receptor(green)aredetectedinneuron-shapedcells(arrows).StainingsuggestspartialcolocalizationbetweenCaV1.2andGABA(A)α1Receptorinasub-populationofdentategyrusneurons.A.Mousehippocampus.B.Rathippocampus.
GuineapigAnti-CaV1.2(CACNA1C)Antibody(#AGP-001),raisedinguineapigs,isahighlyspecificantibodydirectedagainstanepitopeoftheratprotein.Theantibodycanbeusedinwesternblotandimmunohistochemistryapplications.ItandhasbeendesignedtorecognizeCaV1.2frommouse,ratandhumansamples.TheantigenusedtoimmunizeguineapigsisthesameasAnti-CaV1.2(CACNA1C)Antibody(#ACC-003)raisedinrabbit.Ourlineofguineapigantibodiesenablesmoreflexibilitywithourproductssuchasimmuno-colocalizationstudies,immunoprecipitation,etc.
ebiomall.com
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多谢!
不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!
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