Thisproductisfreezedried.Allwatermoleculeshavebeenremoved.
Everylotistried&testedinarelevantBIOLOGicalassay.
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- Liao,C.F. etal. (1989) J.Biol.Chem. 264, 7328.
- Lazareno,S. etal. (1990) Br.J.Pharmacol. 109, 1120.
- Hernandez,M. etal. (1993) Br.J.Pharmacol. 110, 1413.
- AlomoneLabsTropicamideinhibitscarbachol’seffectonM1mAChRexpressedinC6cells.CellswereloadedwithFluo-3AMandchangesinintracellularCa2+ weredetectedviachangesinFluo-3emission.A.Normalizedfluorescencebeforeandafterapplication(at20seconds,seearrow)of10μMCarbacholfollowinga20minincubationwith0-100μM Tropicamide (#T-120)(asindicated).B.Inhibition(aspercentofcontrol)of10µMCarbacholevokedCa2+ rise,plottedagainstTropicamideconcentrations.
- 1.Bonner,T.I. etal. (1989) Trends.Neurosci. 12, 148.
- 2.Felder,C.C. etal. (1995) FASEB.J. 9, 619.
- 3.Bonner,T.I. etal. (1987) Science 237, 527.
- 4.Peralta,E.G. etal. (1987) EMBOJ. 6, 3923.
- 5.Bonner,T.I. etal. (1988) Neuron 1, 403.
- 6.Liao,C.F. etal. (1989) J.Biol.Chem. 264, 7328.
- 7.Liao,C.F. etal. (1989) J.Biol.Chem. 264, 7328.
- 8.Lazareno,S. etal. (1990) Br.J.Pharmacol. 109, 1120.
- 9.Hernandez,M. etal. (1993) Br.J.Pharmacol. 110, 1413.
- 10.Manny,R.E. etal. (2001) Invest.Ophtalmol.Vis.Sci. 42, 1728.
Muscarinicacetylcholinereceptors(mAChR)regulateanumberofimportantbasicphysiologicfunctionsincludingheartrate,motorandsensorycontrolandmorecomplexbehaviorsincludingarousal,memory,andlearning.LossofmuscarinicreceptornumberorfunctionhasbeenimplicatedintheetiologyofseveralneurologicaldisordersincludingAlzheimer"sdementia,Down"ssyndromeandParkinson"sdisease.
Fivesubtypesofmuscarinicreceptors(m1-m5)havebeenidentifiedbymolecularcloning1andmuchhasbeenlearnedabouttheirdistribution,pharmacology,andstructure2-6.
Tropicamideisamuscarinicreceptorantagonist,whichshowsa3foldselectivityform47-8.ItseffecthasapIC50of~7.5forporcineintravesicaluretercontraction9.
Tropicamideproducesshortactingmydriasis(dilationofthepupil)andcycloplegia(paralysisoftheciliarymuscle)whenappliedaseyedrops.Itisalsoaneffectivecycloplegicagentinmyopicchildren10.
Tropicamide(#T-120) isahighlypure,synthetic,andbiologicallyactivecompound.
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多谢!
不知道能不能直接买到大鼠抗豚鼠血管IgG的ELISA试剂盒。
如果买不到这种试剂盒,用空白板的话(就是反应孔中没有抗原或者抗体包被),把自制的豚鼠血管抗原加到反应孔中进行孵育,能不能使抗原附着在孔壁上?这样的话就可以按照elisa的操作步骤进行大鼠血清检测了。
目前的设想是自制抗原,粉碎豚鼠的血管,制成悬液,通过反复多次的冻融,离心后收集上清液作为抗原(即豚鼠血管抗原)。
然后将抗原加入到elisa的反应孔中(这种是特制的空白板,就是反应孔中没有抗原或抗体包被)进行孵育,使豚鼠血管抗原附着在孔壁上,就是让反应孔充当固相载体,形成固相抗原。倒掉多余的抗原。
再加入待检测的大鼠血清,这样血清中的特异性抗豚鼠血管IgG就可以跟固相的抗原结合,形成固相抗原抗体复合物。
加入酶标的兔抗鼠或者羊抗鼠IgG,形成酶标的抗原抗体复合物。
然后就是一些显色步骤。
现在关键的问题就是抗原加入空白板,能不能形成固相抗原,如果不能跟孔壁附着的话,在后面洗涤的过程中就被洗掉了,那就没办法完成检测了。
请各位大侠给指条明路啊!!!小弟在此多谢了!!!
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