Product Name | SensoLyte ® Homogeneous AFC Caspase - 3/7 Assay Kit500 assays (96 - well plate) |
Size | 1 kit |
Catalog # | AS-71114 |
US$ | $315 |
Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors. | |
Detailed Information | DatasheetMaterial Safety Data Sheets (MSDS)Poster |
Product Citations | Freise, C. & U. Querfeld(2014). Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34. Pharmacol Res doi: http://dx.doi.org/10.1016/j.phrs.2014.04.013.Johnson, JG. et al. (2014). Caspase-like activity during aging and cell death in the toxic dinoflagellate< i> Karenia brevis. Harmful Algae 31, 41.Meek, RL. et al. (2013). Glomerular cell death and inflammation with high-protein diet and diabetes. Nephrol Dial Transplant doi: 10.1093/ndt/gfs579.Freise, C. et al. (2013). The inhibitor of Ca2+-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB. Invest New Drugs. doi:10.1007/s10637-012-9879-6Freise, C. et al. (2011). A hepatoprotective Lindera obtusiloba extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines. BMC Complementary Altern Med 11, 39.Cheung, M. et al. (2010). An evolved ribosome-inactivating protein targets and kills human melanoma cells in vitro and in vivo. Mol Cancer 10.1186/1476-4598-9-28.Freise, C. et al. (2009). An active extract of Lindera obtusiloba inhibits adipogenesis via sustained Wnt signaling and exerts anti-inflammatory effects in the 3T3-L1 preadipocytes. J Nutr Biochem 10.1016/j.jnutbio.2009.09.013.Rueh, M. et al. (2009). Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-β-mediated profibrotic gene expression pattern. J Nutr Biochem 20, 597.Ou, D. et al. (2005). Synergistic inhibition of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in human pancreatic β cells by Bcl-2 and X-linked inhibitor of apoptosis. Hum Immunol 66, 274. |
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电感多用于电源滤波回路,磁珠多用于信号回路,用于EMC对策磁珠主要用于抑制电磁辐射干扰,而电感用于这方面则侧重于抑制传导性干扰。两者都可用于处理EMC、EMI问题。磁珠是用来吸收超高频信号,象一些RF电路,PLL,振荡电路,含超高频存储器电路(DDR SDRAM,RAMBUS等)都需要在电源输入部分加磁珠,而电感是一种蓄能元件,用在LC振荡电路,中低频的滤波电路等,其应用频率范围很少超过错50MHZ。地的连接一般用电感,电源的连接也用电感,而对信号线则采用磁珠?
但实际上磁珠应该也能达到吸收高频干扰的目的啊?而且电感在高频谐振以后都不能再起电感的作用了,先必需明白EMI的两个途径,即:辐射和传导,不同的途径采用不同的抑制方法。前者用磁珠,后者用电感。对于扳子的IO部分,是不是基于EMC的目的可以用电感将IO部分和扳子的地进行隔离,比如将USB的地和扳子的地用10uH的电感隔离可以防止插拔的噪声干扰地平面?电感一般用于电路的匹配和信号质量的控制上。在模拟地和数字地结合的地方用磁珠。在模拟地和数字地结合的地方用磁珠。数字地和模拟地之间的磁珠用多大,磁珠的大小(确切的说应该是磁珠的特性曲线),取决于你需要磁珠吸收的干扰波的频率,为什么磁珠的单位和电阻是一样的呢??都是欧姆!!磁珠就是阻高频嘛,对直流电阻低,对高频电阻高,不就好理解了吗, 比如1000R@100Mhz就是说对100M频率的信号有1000欧姆的电阻,因为磁珠的单位是按照它在某一频率产生的阻抗来标称的,阻抗的单位也是欧姆。磁珠的datasheet上一般会附有频率和阻抗的特性曲线图。一般以100MHz为标准,比如2012B601,就是指在100MHz的时候磁珠的Impedance为600欧姆。
2、检测电感量,电阻及阻抗,那要看客户的要求。
3、磁珠是有电感值要求的。 电感值的高低 代表了磁导率的高低。电感值越大,阻抗越高。(前提是磁芯的磁导率高频衰减小,阻抗就高)
电阻 电子元器件(resistor):是一种纯阻抗,对任何频率的体现的阻值都一样,不随频率变化而变化。
我们公司也是做磁珠的
还不错
不妨可以联系下我们
以常用于电源滤波的 HH-1H3216-500为例,其型号各字段含义依次为:HH 是其一个系列,主要用于电源滤波,用于信号线是HB系列;1 表示一个组件封装了一个磁珠,若为4则是并排封装四个的;
H 表示组成物质,H、C、M为中频应用(50-200MHz),T低频应用50MHz),S高频应用(200MHz);
3216 封装尺寸,长3.2mm,宽1.6mm,即1206封装;
500 阻抗(一般为100MHz时),50 ohm。其产品参数主要有三项:
阻抗[Z]@100MHz (ohm) : Typical 50, Minimum 37;直流电阻 DC esistance (m ohm): Maximum 20;
额定电流 Rated Current (mA): 2500。
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