Source | M.W. | CAS No. | |||
---|---|---|---|---|---|
Structural Info | |||||
Formulation | Lyophilized in sterile filtered solution of PBS. | ||||
Reconstitution | Before reconstitution, a brief spin is recommend to drive down anymaterial dislodged from the bottom of the tube. The lyophilized proteinshould be reconstituted in sterile H2O to a desired concentration. | ||||
Stability | The lyophilized protein is stable for at least one year if stored at -80°C. Reconstituted protein is stable for at least four weeks at 4 °C, butshould be stored in aliquots at -80 °C for longer term. Avoidrepeated freeze and thaw. | ||||
Purity | Greater than 90% as determined by SDS-PAGE analysis | ||||
Biological Activity | The activity was determined by using a TCF reporter gene assay in cultured human cells. The EC50 ranges from 2 - 10 ng/ml in the presence of 10 ng/mL human WNT-3a. | ||||
Country of Origin | USA |
R Spondin-2 is a natural enhancer of the canonical WNT pathway. Whenused together with WNT proteins that activate the beta-catenin pathway, Rspondin-1 enhances the activity of canonical WNT proteins by binding toLGR5 and LGR4 (refs.)Refs: de Lau, et al, LGR5 homologues associate with Wnt receptors and mediate R-spondinsignaling. Nature. 2011 July 4; 476: 293; Carmon, et al, R-spondins function as ligandsfor the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling. PNAS.2011 Jul 12; 108: 11452.
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308C是16孔道的,单次反应可检测1-14项不同指标。
要做滚环扩增,实验室没有荧光光谱仪,可以用荧光定量PCR仪代替吗?
(1)水浴式PCR仪:变温快、时间准、温度均一,但体积大,自动化程度不高。
(2)变温金属块式PCR仪:通过半导体加热和冷却,并由微机控制恒温和冷热处理过程。装置比较牢固耐用,温度变换平稳,有利于保持Taq DNA聚合酶的活性。
(3)变温气流式PCR仪:以空气作为热传播媒介,由大功率风扇及制冷设备提供外部冷空气而制冷。成本较低;安全程度高。微机配上软件,可灵活编程。
求Hema9600基因扩增仪用户手册,实验室有这个仪器,官网(http://www.zhhema.com/zh-CN/displayproduct.html?proID=100438154)找不到说明书。
暂无品牌问答