DescriptionViPrimePLUS One Step Taq RT-qPCR Green Master Mix I is next generation master mix designed for one step real-time PCR reaction set up. The master mix is prepared in 2X concentrated solution and contains unique thermostable M-MULV enzyme, Taq DNA Polymerases, SYBR® Green dye as well as MgCl2 and buffer components at optimal concentrations. The MMULV enzyme has an optimal operating temperature and a higher affinity for primer template duplexes which allows very rapid processing during RT step. SYBR® Green dye emits fluorescence when bound to doublestranded DNA. Detection of PCR product is monitored by the increase in fluorescence, leading to high sensitivity, wide dynamic range and high reproducibility for quantification.ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The formulation of RT-qPCR master mix can detect low copy number targets very specifically with high efficiency that give CT values close to the theoretical time of detection. The ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The master mix provides convenient and robust set up for quantitative real-time analysis of RNA samples.ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I has several formulations optimized to be used with most of real-time PCR instruments. The sensitivity and consistency of ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I in standard cycling conditions gives the industry leading performance in fast cycling conditions.
ApplicationsAll kinds of RNA sample material suited for RT-qPCR amplification can be used.
Features
- One step real time RT-qPCR reaction set up
- Equipped with thermostable M-MULV enzyme and SYBR® Green dye for intercalator-based qPCR
- Good buffer system for excellent amplification efficiency
- High sensitivity detection
- Optimal performance for highly sensitive and specific RT-qPCR reaction
- Compatible with most of the real-time PCR platforms
Component3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”
StorageStable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.
Quality ControlAs part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.
Limitation Of UseFor research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.
InstrumentsTo calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.
| Product Description | Compatible Hardware |
| QLMM14ViPrimePLUS One Step Taq RT-qPCR Green Master Mix I (SYBR® Green Dye) | Biometra qTower, BioRad iCycler, BioRad IQ4, BioRad IQ5, Cepheid SmartCycler®, Eppendorf Mastercycler, Fluidigm BioMark™, Illumina Eco, MJ Chromo4, Opticon, PCRMax Eco™, Roche lightcycler® 480, lightcycler® LC96 and lightcycler® Nano Platforms, RotorGene, Roche Capillary Lightcycler 1.0-2.0, Stratagene MX MX4000P®, MX3000P®, MX3005®, Thermo PikoReal™ |
| QLMM14-LRViPrimePLUS One Step Taq RT-qPCR Green Master Mix I with Low ROX (SYBR® Green Dye) | Applied Biosystems 7500 and 7500 FAST platform, QuantStudio™, ViiA7 |
| QLMM14-RViPrimePLUS One Step Taq RT-qPCR Green Master Mix I with ROX (SYBR® Green Dye) | Applied Biosystems 7000, 7300, 7700, 7900 and 7900HT FAST platforms, GeneAmp® 5700, StepOne™, StepOne™ PLUS |
Ordering Information
| Catalog No | Description | Pack Size |
| QLMM14 | ViPrimePLUS One Step Taq RT-qPCR Green MasterMix I (SYBR®Green Dye) | 150 reactions |
| QLMM14-LR | ViPrimePLUS One Step Taq RT-qPCR Green MasterMix I with Low ROX (SYBR® Green Dye) | 150 reactions |
| QLMM14-R | ViPrimePLUS One Step Taq RT-qPCR Green MasterMix I with ROX (SYBR® Green Dye) | 150 reactions |
DownloadManual
ViPrimePLUS One Step Taq RT-qPCR Green MasterMix I (SYBR®Green Dye) ViPrimePLUS One Step Taq RT-qPCR Green MasterMix I with Low ROX (SYBR® Green Dye) ViPrimePLUS One Step Taq RT-qPCR Green MasterMix I with ROX (SYBR® Green Dye)
PublicationThis Product Has Been Used In: Yousef, M.I. et al. (2019)Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles, . Journal of Taibah University for Science, 13:1,570-578 Yousef, M.I. et al. (2019) Hepato-renal toxicity of oral sub-chronic exposure to aluminum oxide and or zinc oxide nanoparticles in rats, . Toxicology Reports, , 336-346.El Serafy, M.A., Sabry, D., El din, M. A. M., Moustafa, A., El Kazaz, A., Mohammed, S., R., Abou-Elalla, A.A. (2017). Association of SIRT-1, T helper 17-associated gene and Antimicrobial Peptides Genes in Inflammatory Bowel Disease Patients. . . International Journal of Biomedicine. Vol. 7, No. 3 (2017) 196-203
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
308C是16孔道的，单次反应可检测1-14项不同指标。
要做滚环扩增，实验室没有荧光光谱仪，可以用荧光定量PCR仪代替吗？
（1）水浴式PCR仪：变温快、时间准、温度均一，但体积大，自动化程度不高。
（2）变温金属块式PCR仪：通过半导体加热和冷却，并由微机控制恒温和冷热处理过程。装置比较牢固耐用，温度变换平稳，有利于保持Taq DNA聚合酶的活性。
（3）变温气流式PCR仪：以空气作为热传播媒介，由大功率风扇及制冷设备提供外部冷空气而制冷。成本较低；安全程度高。微机配上软件，可灵活编程。
求Hema9600基因扩增仪用户手册，实验室有这个仪器，官网（http://www.zhhema.com/zh-CN/displayproduct.html?proID=100438154）找不到说明书。
暂无品牌问答
