Description:
ChromogenixCoamatic®Heparin isachromogenicassaykitforthequantitativedeterminationofunfractionatedheparin(UFHeparin)orlowmolecularweightheparin(LMWHeparin)inhumancitratedplasmausingautomatedandmicroplatemethods.
HeparinKitComposition:
Reagents, Packaging,Storage,StABIlityThesealedreagentsarestableat2-8°Cuntiltheexpirydateprintedonthelabel.
- S-2732™,15mg,2vials.Chromogenicsubstrate,Suc-Ile-Glu(-pip)-Gly-Arg-pNA·HCllyophilizedwithdetergentandmannitolasbulkingagent.Stabilityafterreconstitution:3monthsat+2-8°Cintheoriginalvial.Avoidexposuretolight.Discardthesubstratesolutionifitappearsyellow.Avoidcontaminationbymicroorganisms.
- FactorXa,35nkat,2vials.LyophilizedbovineFXacontainingTrisbuffer,EDTA,NaCl,dextransulfateandbovineserumalbumin.Stabilityafterreconstitution:3monthsat+2-8°Cintheoriginalvial.
MeasurementPrinciple:
[AT •Heparin] | [FXa •AT •Heparin] | ||
+FXa | → | ||
S-2732™ | Peptide+pNA |
FactorXa(FXa)isaddedtoamixtureofundilutedplasmaandthechromogenicsubstrateS-2732™.WhenHeparinandATarecomplexed,twocompetingreactionsoccursimultaneously:
- InhibitionofFXabythe[AT·Heparin]complex.
- ReactionofFXawithS-2732™resultingincleavageofpNA.ThepNAreleasemeasuredat405nmisinverselyproportionaltotheheparinlevelinthesample.InordertoreducetheinfluencefromheparinantagoNISTs,suchasplateletfactor4(PF4),dextransulfateisincludedinthereactionmixture.
Background:
Heparinisthemostfrequentlyusedantithrombotictherapeutic.TheBIOLOGicalactivityofthissulfatedglycosaminoglycanresidesinitsabilitytoaccelerate(upto2000-fold)theinhibitoryeffectofantithrombin(AT)onthecoagulationproteases.TheamountofLMWHeparinorUFHeparinisdeterminedfromtheanti-FXaactivityexpressedbythe[AT·Heparin]complexformedinplasma.
Coamatic©Heparinmeasurestheabilityofheparintocatalyzetheinhibition ofFXabyantithrombin.Theanti-Xaassayisthemethodofchoicewhen monitoringLMWheparintherapyandisalsosuitableasareplacementfor theAPTTtestwhenmonitoringtherapywithUFHeparin.
SimplifiedHeparintesting,undilutedassaywithsuperiorreagentstability.
Readmoreaboutheparin.
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bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
通过解答,教会我,谢谢
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
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