Description:
Technozym®Glu-PlasminogenisahighlysensitivecompletesandwichELISAfortheselectivedeterminationofGlu-Plasminogeninhumanplasma.TheGlu-PlasminogenELISAkitisbasedontwomonoclonalantibodies;acatchingantibodyandadetectingantibodyspecificfortheGlu-formofPlasminogen. TheGlu-plasminogenELISAisusefulforthedeterminationofGluplasminogenlevels,particularlyinstudiesregardingthroxmboembolicresearch,lyticresearchorinresearchstudiesofhyperfibrinolysis.
Specificity:
ThemonoclonalantibodiesemployedinthistestsystemrecognizeonlyuncleavedGlu-plasminogen.Thus,theresultsarenotaffectedbythepresenceofplasmin-alpha-2-antiplasmincomplexesorplasmin-modifiedLys-plasminogen.TheassaymeasuresGlu-plasminogeninarangefrom0.06-0.5micrograms/milliliter.Normalplasmalevelsare60-250micrograms/milliliter.Theinter-andintra-assayvariationsarelessthan10%and5%,respectively.
KitComposition:
KitComposition
- Plate+PlateCover
12x8wellmicrotitrestripsprecoatedwitha monoclonalanti-Plasminogenantibodyandblocked with1%bovineserumalbumin(BSA),lyophilized.
(TC-CodeGX) - Standard
1xlyophilizedNormalPlasma
(TC-CodeBJ) - POX-Antibody
1xconjugatedpolyclonalantiPlasminogenantibodies(concentrated).
(TC-CodeBG) - IncubationBuffer
(PBS;pH7,3);containsstABIlizerprotein;0.05%proclin;and bluedye,1bottle,90ml,readyforuse
(TC-CodeNB) - Substrate–(greencap)
1x12mlTMB(Tetramethylbenzidine)insubstratebuffercontaining H2O2.Readytouse.
(TC-CodeKN) - StopSolution–(redcap)
1x12ml0.45mol/lSulphuricAcid
(TC-CodeKK) - WashingBufferConcentrate-(1+11,5)
(PBS;pH7,3)containingdetergent;0,01%merthiolat,1bottle,80ml
(TC-CodeNA)
Kitstorage:Storeallcomponentsat2-8°C.
MaterialsRequired(butnotsupplied)
- MicroPipettesandamultichannelmicropipette;pipettetips.
- Glassorplastictesttubesfordilutingthestandard+samples.
- Laboratorybottlesorbeakersandgraduatedcylindersfordilutingwash andincubationbuffer.
- Distilledordeionizedwater.
- Absorbentpapertowels.
- Microtitreplatewasher(alternatively,washingcanbeperformed manuallyusingamultichannelpipetteorrepeatingsyringe).
- Amicrotitreplatereaderequippedwitha450nmfilterand,ifpossIBLe,a 620nmreferencefilter.
- A37°Cincubator.
- Graphpaper.
MeasurementPrinciple:
TheTCGlu-plasminogenELISAtestisasolidphaseenzyme immunoassay.
Background:
Plasminogenistheinactiveprecursorofplasmin,thecentralenzyme responsibleforfibrinolysis.Plasminogen,whichissynthesizedbytheliver, isa92,000daltonsingle-chainglycoprotein.Itcirculatesinplasmaata concentrationofapproximately200µgpermlwithahalflifeof2.2days. Throughtheactionofcertainproteasessuchastissue-typeplasminogen activatororurokinase,asinglepeptidebondintheplasminogenmolecule iscleavedtoyieldthetwochainmolecule,plasmin.Plasmin’smajor functionisphysiologicclotdissolutionthroughthedegradationoftheinsoluble polymerfibrinintosolublefragments.Thus,theconcentrationof plasminogenisoneofthenumerousfactorscriticallyinfluencingtherateof fibrinolysisinvivo.
Plasminogenlevelsareoftendepressedinbothacuteandchronichepatic disease.Thismaybedueeithertodecreasedsynthesisorincreased consumptionduringdisseminatedintravascularcoagulation.Inprimary biliarycirrhosisorearlycommonbileductobstructionplasminogenvalues arenormalorevenelevated.Abnormallylowplasminogenvalueshave beenfoundinpatientswithgeneralizedhyperfibrinolysisandneonateswith Lipströmsyndrome.
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
请问有什么方法检测细胞内活性氧更好,各位有什么经验,及相关试剂盒推荐一下。
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
请问下有无同学需要H37RA的?我是做EAE模型的,上个月购买了BDDifco公司的H37RA(货号),因为购买的时候只能整盒6支购买,但我们用不了那么多,所以想问问有无同学需要的,100mg/支,800元/支或用等价试剂交换。地址广州。有需要的请私信,谢谢!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
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