Description:
TheREAADSAnti-Phosphatidylserine(aPS)IgG/IgMtestkitisaindirect,semi-quantitativeELISAusedtodetectantibodiesagainstphosphatidylserine.EachassayusesasingleormultipointcalibratortoobtainIgG/IgManti-phosphatidylserineantibodicconcentrationsfrompatientsamples.
Advantages:
- ConvenientELISAProcedure
- ForthespecificdeterminationofIgG/IgMisotypes
- Objective,accurateandreproducIBLe
- Reagentcompletekits
- Totalincubationtime:40minutesatroomtemperature
- EasilyAutomated
- Choiceofsingleormulti-pointcalibration
PrincipleandProcedure:
Principle
TheREAADSIgG,IgMandIgAAnti-Phosphatidylserine(aPS)TestKitsareindirect,semi-quantitativeELISAsusedtodetectantibodiesagainstphosphatidylserine.EachassayusesasingleormultipointcalibratortoobtainIgG,IgMorIgAanti-phosphatidylserineantibodyconcentrationsfrompatientsamples.
Procedure
Patientserumisdilutedintosamplediluentcontainingbovineserum(sourceofcofactor)andincubatedinwellscoatedwithphosphatidylserine.AnyaPSantibodiespresentinthepatientsamplewillbindtothecoatedwells.Afterremovalofunboundserumbywashing,antibodiesspecificforhumanIgG,IgMorIgAlabeledwithhorserADIshperoxidase(HRP),areaddedtoformcomplexeswiththephosphatidylserineboundantibodies.
Thewellsarewashed,andachromogenicsubstrateisadded,resultinginacolorchangethatisproportionaltotheamountofantibodypresent.Theassayisstoppedbytheadditionofastoppingsolution.ResultsareobtainedbyreadingtheO.D.(opticaldensity)ofeachwellat450nminaspectrophotometer.TheO.D.valuesofcontrolandpatientsamplesaremultipliedbythecalibratorconversionfactortoobtainIgG,IgMorIgAanti-phosphatidylserineantibodyconcentrationsinGPS,MPSorAPSunits,respectively.Assaycutoffshavebeenestablishedat16GPS,22MPSand20APSunits.
ClinicalPerformance:
ClinicalSpecificity
Serumsamplesfrommultiplehealthyblooddonorpopulationswereassayedforthepresenceofantiphosphatidylserineantibodies.Specificitywasshowntobe96%forbothIgGandIgMantibodies,and95%forIgA.
ClinicalSensitivity
UnselectedSLEsamplesweretestedtodeterminetheclinicalsensitivityoftheaPSassay.Theresultingsensitivitywas22.2%forIgG,25%forIgMand11.1%forIgA.TheclinicalsensitivityoftheassayforAntiphospholipidSyndrome(APS)wasdeterminedbycomparingaPStestresultsfromtwogroupsofselectedpatients:
Group1–PrimaryAPS | IgGaPS | IgMaPS | IgAaPS |
Assaycutoff | 16GPS | 22MPS | 20APS |
Averagevalue | 61.6GPS | 33.4MPS | 21.1APS |
%positive | 90.9% | 54.5% | 36.4% |
Group2–SLEcontrol | IgGaPS | IgMaPS | IgAaPS |
Assaycutoff | 16GPS | 22MPS | 20APS |
Averagevalue | 9.2GPS | 9.9MPS | 11.2APS |
%positive | 11.1% | 11.1% | 0% |
- Group1–patientswithPrimaryAPS
- Group2–SLEpatientswithnohistoryofthrombosisorthrombocytopenia(controls).Theresultsareshowninthechartabove.
TheprevalenceandmeanlevelsofaPSantibodieswerehigherinPrimaryAPSpatientsthanintheSLEcontrolgroup.Theseassays,whenruninconcertwithadditionalanti-phospholipidandlupusanticoagulantassays,providesamorecompleteprofileforestablishingadiagnosisofAntiphospholipidSyndromeandforassessingtheriskofthromboticcomplicationsinSLEpatients.
Background:
Elevatedserumlevelsofanti-phospholipidantibodiesareassociatedwithanincreasedriskforrecurrentarterialandvenousthromboticevents,thrombocytopeniaandfetalloss.ThesearethemainclinicalmanifestationsofAntiphospholipid
Syndrome.Phosphatidylserineisfoundinthemembranesofplateletsandendothelialcells,whichparticipateinthecoagulationcascade.Duetoitsphysiologicrole,testingforautoantibodiesdirectedtowardsphosphatidylserineprovidesnotonlymorerelevantresults,butalsoadditionalinformationtoassesstheriskofthrombosis.
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bhclna2so4nano3na2co3
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dba(oh)2nahco3alcl3nahso4
通过解答,教会我,谢谢
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
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